Direct estimation of the recombination frequency between the RB1 gene and two closely linked microsatellites using sperm typing.
Details
Serval ID
serval:BIB_9B640472CA16
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Direct estimation of the recombination frequency between the RB1 gene and two closely linked microsatellites using sperm typing.
Journal
European Journal of Human Genetics
ISSN
1018-4813
Publication state
Published
Issued date
1999
Peer-reviewed
Oui
Volume
7
Number
2
Pages
239-242
Language
english
Abstract
In this study, single sperm typing has been used for high-resolution recombination analysis between the retinoblastoma gene and two closely linked extragenic microsatellites (D13S284 and D13S1307). The analysis of 1198 single sperm from three donors allowed the determination of recombination fractions between RB1.20 and D13S284 and RB1.20 and D13S1307 of 0.022 and 0.033, respectively. These results show that RB1 gene and the two microsatellites are closely linked, which validates their potential use in indirect genetic diagnosis of retinoblastoma.
Keywords
Humans, Linkage (Genetics), Male, Microsatellite Repeats, Recombination, Genetic, Retinoblastoma Protein/genetics, Spermatozoa
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 13:54
Last modification date
20/08/2019 16:02
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