Competitor analogs for defined T cell antigens: peptides incorporating a putative binding motif and polyproline or polyglycine spacers

Details

Serval ID
serval:BIB_9B5193EABEA2
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Competitor analogs for defined T cell antigens: peptides incorporating a putative binding motif and polyproline or polyglycine spacers
Journal
Cell
Author(s)
Maryanski  J. L., Verdini  A. S., Weber  P. C., Salemme  F. R., Corradin  G.
ISSN
0092-8674 (Print)
Publication state
Published
Issued date
01/1990
Volume
60
Number
1
Pages
63-72
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan 12
Abstract
We describe a new approach for modeling antigenic peptides recognized by T cells. Peptide A24 170-182 can compete with other antigenic peptides that are recognized by H-2kd-restricted cytolytic T cells, presumably by binding to the Kd molecule. By comparing substituted A24 peptides as competitors in a functional competition assay, the A24 residues Tyr-171, Thr-178, and Leu-179 were identified as possible contact residues for Kd. A highly active competitor peptide analog was synthesized in which Tyr was separated from the Thr-Leu pair by a pentaproline spacer. The choice of proline allowed the prediction of a probable conformation for the analog when bound to the Kd molecule. The simplest conformation of the A24 peptide that allows the same spacing and orientation of the motif as in the analog would be a nearly extended polypeptide chain incorporating a single 3(10) helical turn or similar structural kink.
Keywords
Amino Acid Sequence Animals Binding, Competitive Cells, Cultured Clone Cells Cytotoxicity, Immunologic *Glycine HLA Antigens/*immunology Major Histocompatibility Complex Mice Mice, Inbred DBA Models, Molecular Molecular Sequence Data Oligopeptides/chemical synthesis/immunology/*pharmacology *Proline Protein Conformation Structure-Activity Relationship T-Lymphocytes, Cytotoxic/*immunology
Pubmed
Web of science
Create date
24/01/2008 14:55
Last modification date
20/08/2019 15:02
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