Insulin secretion from human beta cells is heterogeneous and dependent on cell-to-cell contacts.

Details

Serval ID
serval:BIB_9B39AFC6F19D
Type
Article: article from journal or magazin.
Collection
Publications
Title
Insulin secretion from human beta cells is heterogeneous and dependent on cell-to-cell contacts.
Journal
Diabetologia
Author(s)
Wojtusciszyn A., Armanet M., Morel P., Berney T., Bosco D.
ISSN
0012-186X (Print)
ISSN-L
0012-186X
Publication state
Published
Issued date
10/2008
Peer-reviewed
Oui
Volume
51
Number
10
Pages
1843-1852
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
We assessed the heterogeneity of insulin secretion from human isolated beta cells and its regulation by cell-to-cell contacts.
Insulin secretion from single and paired cells was assessed by a reverse haemolytic plaque assay. The percentage of plaque-forming cells, the mean plaque area and the total plaque development were evaluated after 1 h of stimulation with different secretagogues.
Not all beta cells were surrounded by a haemolytic plaque under all conditions tested. A small fraction of the beta cell population (20%) secreted more than 90% and 70% of total insulin at 2.2 and 22.2 mmol/l glucose, respectively. Plaque-forming cells, mean plaque area and total plaque development were increased at 12.2 and 22.2 compared with 2.2 mmol/l glucose. Insulin secretion of single beta cells was similar at 12.2 and 22.2 mmol/l glucose. Insulin secretion of beta cell pairs was increased compared with that of single beta cells and was higher at 22.2 than at 12.2 mmol/l glucose. Insulin secretion of beta cells in contact with alpha cells was also increased compared with single beta cells, but was similar at 22.2 compared with 12.2 mmol/l glucose. Delta and other non-beta cells did not increase insulin secretion of contacting beta cells compared with that of single beta cells. Differences in insulin secretion between 22.2 and 12.2 mmol/l glucose were observed in murine but not in human islets.
Human beta cells are highly heterogeneous in terms of insulin secretion so that a small fraction of beta cells contributes to the majority of insulin secreted. Homologous and heterologous intercellular contacts have a significant impact on insulin secretion and this could be related to the particular architecture of human islets.
Keywords
Animals, Cell Communication/physiology, Glucose/metabolism, Humans, Immunohistochemistry, Insulin/metabolism, Insulin Secretion, Insulin-Secreting Cells/cytology, Insulin-Secreting Cells/metabolism, Islets of Langerhans/cytology, Islets of Langerhans/metabolism, Male, Mice
Pubmed
Web of science
Open Access
Yes
Create date
14/06/2021 9:59
Last modification date
24/05/2024 13:16
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