Regulation of interleukin-1ra, interleukin-1 alpha, and interleukin-1 beta production by human alveolar macrophages with phorbol myristate acetate, lipopolysaccharide, and interleukin-4.

Details

Serval ID
serval:BIB_9B1DCED6D69A
Type
Article: article from journal or magazin.
Publication sub-type
Case report (case report): feedback on an observation with a short commentary.
Collection
Publications
Title
Regulation of interleukin-1ra, interleukin-1 alpha, and interleukin-1 beta production by human alveolar macrophages with phorbol myristate acetate, lipopolysaccharide, and interleukin-4.
Journal
American Journal of Respiratory Cell and Molecular Biology
Author(s)
Galve-de Rochemonteix B., Nicod L.P., Chicheportiche R., Lacraz S., Baumberger C., Dayer J.M.
ISSN
1044-1549[print], 1044-1549[linking]
Publication state
Published
Issued date
1993
Volume
8
Number
2
Pages
160-168
Language
english
Abstract
Human alveolar macrophages (AM) are antigen-presenting cells that have an important immune effector function in the lung. We have previously shown that AM produce a specific interleukin-1 (IL-1) inhibitor of 20 to 25 kD that blocks biologic activities of IL-1 alpha and IL-1 beta such as prostaglandin E2 production by fibroblasts. This inhibitor acts as a receptor antagonist (IL-1ra) by binding to the IL-1 receptor. We are now presenting evidence that the natural AM-derived IL-1ra is immunologically identical to IL-1ra cloned from human peripheral blood monocytes and shows a band at 20 kD compatible with the natural glycosylated IL-1ra. No constitutive expression of IL-1 mRNA was detected when analyzed by Northern blot immediately after bronchoalveolar lavage from six control patients. Comparison of in vitro kinetics of IL-1ra, IL-1 alpha, and IL-1 beta analyzed during culture in the presence or absence of phorbol myristate acetate revealed that their mRNA expression was asynchronous. IL-1 alpha and IL-1 beta mRNA were expressed after as little as 15 min, whereas IL-1ra mRNA was detectable only after 3 h in culture. The production of IL-1ra was measured by enzyme-linked immunosorbent assay and compared with that of IL-1 alpha and IL-1 beta. In freshly isolated AM (10(6)/ml), cell-associated IL-1ra was present in an average amount of 2.0 +/- 0.5 ng/ml, i.e., 25 and 100 times more than IL-1 alpha and IL-1 beta, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
Keywords
Blotting, Western, Cells, Cultured, Humans, Interleukin-1/biosynthesis, Interleukin-1/genetics, Interleukin-4/pharmacology, Kinetics, Lipopolysaccharides/pharmacology, Macrophages, Alveolar/drug effects, Macrophages, Alveolar/metabolism, RNA, Messenger/metabolism, Receptors, Interleukin-1/antagonists & inhibitors, Tetradecanoylphorbol Acetate/pharmacology, Up-Regulation/drug effects
Pubmed
Web of science
Create date
23/02/2010 14:56
Last modification date
20/08/2019 15:02
Usage data