An OPR3-independent pathway uses 4,5-didehydrojasmonate for jasmonate synthesis.

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Version: Author's accepted manuscript
Serval ID
serval:BIB_9B08CC921BC3
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
An OPR3-independent pathway uses 4,5-didehydrojasmonate for jasmonate synthesis.
Journal
Nature chemical biology
Author(s)
Chini A., Monte I., Zamarreño A.M., Hamberg M., Lassueur S., Reymond P., Weiss S., Stintzi A., Schaller A., Porzel A., García-Mina J.M., Solano R.
ISSN
1552-4469 (Electronic)
ISSN-L
1552-4450
Publication state
Published
Issued date
02/2018
Peer-reviewed
Oui
Volume
14
Number
2
Pages
171-178
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Biosynthesis of the phytohormone jasmonoyl-isoleucine (JA-Ile) requires reduction of the JA precursor 12-oxo-phytodienoic acid (OPDA) by OPDA reductase 3 (OPR3). Previous analyses of the opr3-1 Arabidopsis mutant suggested an OPDA signaling role independent of JA-Ile and its receptor COI1; however, this hypothesis has been challenged because opr3-1 is a conditional allele not completely impaired in JA-Ile biosynthesis. To clarify the role of OPR3 and OPDA in JA-independent defenses, we isolated and characterized a loss-of-function opr3-3 allele. Strikingly, opr3-3 plants remained resistant to necrotrophic pathogens and insect feeding, and activated COI1-dependent JA-mediated gene expression. Analysis of OPDA derivatives identified 4,5-didehydro-JA in wounded wild-type and opr3-3 plants. OPR2 was found to reduce 4,5-didehydro-JA to JA, explaining the accumulation of JA-Ile and activation of JA-Ile-responses in opr3-3 mutants. Our results demonstrate that in the absence of OPR3, OPDA enters the β-oxidation pathway to produce 4,5-ddh-JA as a direct precursor of JA and JA-Ile, thus identifying an OPR3-independent pathway for JA biosynthesis.
Keywords
Alleles, Alternaria, Animals, Arabidopsis/metabolism, Arabidopsis Proteins/metabolism, Biological Assay, Cyclopentanes/metabolism, Disease Resistance, Gene Expression Profiling, Gene Expression Regulation, Plant, Homozygote, Insecta, Isoleucine/analogs & derivatives, Isoleucine/metabolism, Mutation, Oxylipins/metabolism, Plant Diseases/prevention & control, Plant Growth Regulators/metabolism, Plant Roots/metabolism, Signal Transduction
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2018 8:55
Last modification date
20/08/2019 16:02
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