Reduced Renal Colonization and Enhanced Protection by Leptospiral Factor H Binding Proteins as a Multisubunit Vaccine Against Leptospirosis in Hamsters.

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License: CC BY 4.0
Serval ID
serval:BIB_9AF605A168EB
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Reduced Renal Colonization and Enhanced Protection by Leptospiral Factor H Binding Proteins as a Multisubunit Vaccine Against Leptospirosis in Hamsters.
Journal
Vaccines
Author(s)
Techawiwattanaboon T., Barnier-Quer C., Palaga T., Jacquet A., Collin N., Sangjun N., Komanee P., Piboonpocanun S., Patarakul K.
ISSN
2076-393X (Print)
ISSN-L
2076-393X
Publication state
Published
Issued date
22/08/2019
Peer-reviewed
Oui
Volume
7
Number
3
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Subunit vaccines conferring complete protection against leptospirosis are not currently available. The interactions of factor H binding proteins (FHBPs) on pathogenic leptospires and host factor H are crucial for immune evasion by inhibition of complement-mediated killing. The inhibition of these interactions may be a potential strategy to clear leptospires in the host. This study aimed to evaluate a multisubunit vaccine composed of four known leptospiral FHBPs: LigA domain 7-13 (LigAc), LenA, LcpA, and Lsa23, for its protective efficacy in hamsters. The mono and multisubunit vaccines formulated with LMQ adjuvant, a combination of neutral liposome, monophosphoryl lipid A, and Quillaja saponaria fraction 21, induced high and comparable specific antibody (IgG) production against individual antigens. Hamsters immunized with the multisubunit vaccine showed 60% survival following the challenge by 20 LD <sub>50</sub> of Leptospira interrogans serovar Pomona. No significant difference in survival rate and pathological findings of target organs was observed after vaccinations with multisubunit or mono-LigAc vaccines. However, the multisubunit vaccine significantly reduced leptospiral burden in surviving hamsters in comparison with the monosubunit vaccines. Therefore, the multisubunit vaccine conferred partial protection and reduced renal colonization against virulence Leptospira infection in hamsters. Our multisubunit formulation could represent a promising vaccine against leptospirosis.
Keywords
leptospirosis, Leptospira, factor H binding protein, multisubunit vaccine, neutral liposome, MPL, QS21
Pubmed
Web of science
Open Access
Yes
Create date
16/09/2019 22:11
Last modification date
08/08/2024 6:37
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