Effects of cytokines on gonadotropin-releasing hormone (GnRH) gene expression in primary hypothalamic neurons and in GnRH neurons immortalized conditionally
Details
Serval ID
serval:BIB_9AE62F90CE6E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Effects of cytokines on gonadotropin-releasing hormone (GnRH) gene expression in primary hypothalamic neurons and in GnRH neurons immortalized conditionally
Journal
Endocrinology
ISSN
0013-7227 (Print)
Publication state
Published
Issued date
02/2006
Volume
147
Number
2
Pages
1037-43
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb
Research Support, Non-U.S. Gov't --- Old month value: Feb
Abstract
Various cytokines produced during the immune reaction can modulate the neuroendocrine reproductive axis, probably by inducing changes in the activity of hypothalamic GnRH neurons. However, the precise cellular and molecular effects of cytokines on these neurons have not been reported yet. To gain a better insight into these regulations, we first examined the pattern of expression of cytokine receptors in a novel neuronal cell line expressing GnRH (Gnv-4 cells). Among others, gp130 is expressed in Gnv-4 cells, together with the ligand receptor subunits specific for IL-6 as well as oncostatin M (OSM). Consistent with the latter observation, we show that OSM stimulates the expression of the immediate early genes c-fos and early growth response-1 in Gnv-4 cells, an effect dependent upon the activation of the MAPK Erk1/2 intracellular signaling pathway. Functional studies performed in parallel in Gnv-4 cells and in primary hypothalamic neuronal cell cultures show that OSM, although devoid of any effect of its own on GnRH gene expression, can inhibit dose-dependently the stimulation of GnRH expression by N-methyl-d-aspartic acid. In conclusion, these data demonstrate that a GnRH-expressing neuronal cell line can be modulated in vitro by cytokines implicated in the regulation of the reproductive axis. Moreover, they provide the first evidence of an involvement of OSM in these regulations.
Keywords
Analysis of Variance
Animals
Cell Line
Cytokine Receptor gp130/metabolism
Cytokines/*physiology
Gene Expression
Gonadotropin-Releasing Hormone/*genetics/metabolism
Hypothalamus/cytology/*metabolism
Neurons/cytology/*metabolism
Oncostatin M
Rats
Receptors, Interleukin-1/metabolism
Signal Transduction/physiology
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 17:26
Last modification date
20/08/2019 16:02