An Extended, Boolean Model of the Septation Initiation Network in S.Pombe Provides Insights into Its Regulation.

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Ressource 1Download: journal.pone.0134214.PDF (3150.48 [Ko])
State: Public
Version: Final published version
Serval ID
serval:BIB_9AA13CA514FC
Type
Article: article from journal or magazin.
Collection
Publications
Title
An Extended, Boolean Model of the Septation Initiation Network in S.Pombe Provides Insights into Its Regulation.
Journal
Plos One
Author(s)
Chasapi A., Wachowicz P., Niknejad A., Collin P., Krapp A., Cano E., Simanis V., Xenarios I.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Publication state
Published
Issued date
2015
Volume
10
Number
8
Pages
e0134214
Language
english
Abstract
Cytokinesis in fission yeast is controlled by the Septation Initiation Network (SIN), a protein kinase signaling network using the spindle pole body as scaffold. In order to describe the qualitative behavior of the system and predict unknown mutant behaviors we decided to adopt a Boolean modeling approach. In this paper, we report the construction of an extended, Boolean model of the SIN, comprising most SIN components and regulators as individual, experimentally testable nodes. The model uses CDK activity levels as control nodes for the simulation of SIN related events in different stages of the cell cycle. The model was optimized using single knock-out experiments of known phenotypic effect as a training set, and was able to correctly predict a double knock-out test set. Moreover, the model has made in silico predictions that have been validated in vivo, providing new insights into the regulation and hierarchical organization of the SIN.
Pubmed
Web of science
Open Access
Yes
Create date
03/12/2015 16:04
Last modification date
20/08/2019 16:01
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