Durable and controlled depletion of neutrophils in mice.

Details

Serval ID
serval:BIB_9A28486B8033
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Durable and controlled depletion of neutrophils in mice.
Journal
Nature communications
Author(s)
Boivin G., Faget J., Ancey P.B., Gkasti A., Mussard J., Engblom C., Pfirschke C., Contat C., Pascual J., Vazquez J., Bendriss-Vermare N., Caux C., Vozenin M.C., Pittet M.J., Gunzer M., Meylan E.
ISSN
2041-1723 (Electronic)
ISSN-L
2041-1723
Publication state
Published
Issued date
02/06/2020
Peer-reviewed
Oui
Volume
11
Number
1
Pages
2762
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Neutrophils are an essential part of the innate immune system. To study their importance, experimental studies often aim to deplete these cells, generally by injecting anti-Ly6G or anti-Gr1 antibodies. However, these approaches are only partially effective, transient or lack specificity. Here we report that neutrophils remaining after anti-Ly6G treatment are newly derived from the bone marrow, instead of depletion escapees. Mechanistically, newly generated, circulating neutrophils have lower Ly6G membrane expression, and consequently reduced targets for anti-Ly6G-mediated depletion. To overcome this limitation, we develop a double antibody-based depletion strategy that enhances neutrophil elimination by anti-Ly6G treatment. This approach achieves specific, durable and controlled reduction of neutrophils in vivo, and may be suitable for studying neutrophil function in experimental models.
Pubmed
Open Access
Yes
Create date
10/06/2020 20:06
Last modification date
23/10/2020 5:26
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