Differential expression profiles of growth-related genes in the elongation zone of maize primary roots.

Details

Serval ID
serval:BIB_9A04FEEEBB13
Type
Article: article from journal or magazin.
Collection
Publications
Title
Differential expression profiles of growth-related genes in the elongation zone of maize primary roots.
Journal
Plant molecular biology
Author(s)
Bassani M., Neumann P.M., Gepstein S.
ISSN
0167-4412 (Print)
ISSN-L
0167-4412
Publication state
Published
Issued date
10/2004
Peer-reviewed
Oui
Volume
56
Number
3
Pages
367-380
Language
english
Notes
Publication types: Comparative Study ; Journal Article
Publication Status: ppublish
Abstract
Growth in the apical elongation zone of plant roots is central to the development of functional root systems. Rates of root segmental elongation change from accelerating to decelerating as cell development proceeds from newly formed to fully elongated status. One of the primary variables regulating these changes in elongation rates is the extensibility of the elongating cell walls. To help decipher the complex molecular mechanisms involved in spatially variable root growth, we performed a gene identification study along primary root tips of maize (Zea mays) seedlings using suppression subtractive hybridization (SSH) and candidate gene approaches. Using SSH we isolated 150 non-redundant cDNA clones representing root growth-related genes (RGGs) that were preferentially expressed in the elongation zone. Differential expression patterns were revealed by Northern blot analysis for 41 of the identified genes and several candidate genes. Many of the genes have not been previously reported to be involved in root growth processes in maize. Genes were classified into groups based on the predicted function of the encoded proteins: cell wall metabolism, cytoskeleton, general metabolism, signaling and unknown. In-situ hybridization performed for two selected genes, confirmed the spatial distribution of expression shown by Northern blots and revealed subtle differences in tissue localization. Interestingly, spatial profiles of expression for some cell wall related genes appeared to correlate with the profile of accelerating root elongation and changed appropriately under growth-inhibitory water deficit.
Keywords
DNA, Complementary/chemistry, DNA, Complementary/genetics, Expressed Sequence Tags, Gene Expression Profiling, Gene Expression Regulation, Developmental/drug effects, Gene Expression Regulation, Plant/drug effects, In Situ Hybridization, Plant Proteins/genetics, Plant Roots/genetics, Plant Roots/growth & development, RNA, Plant/genetics, RNA, Plant/metabolism, Sequence Analysis, DNA, Water/pharmacology, Zea mays/genetics, Zea mays/growth & development
Pubmed
Web of science
Create date
26/07/2019 16:40
Last modification date
21/08/2019 5:35
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