Hypoxia impairs skin myofibroblast differentiation and function.

Details

Serval ID
serval:BIB_99E5599BCD58
Type
Article: article from journal or magazin.
Collection
Publications
Title
Hypoxia impairs skin myofibroblast differentiation and function.
Journal
The Journal of investigative dermatology
Author(s)
Modarressi A., Pietramaggiori G., Godbout C., Vigato E., Pittet B., Hinz B.
ISSN
1523-1747 (Electronic)
ISSN-L
0022-202X
Publication state
Published
Issued date
12/2010
Peer-reviewed
Oui
Volume
130
Number
12
Pages
2818-2827
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Ischemic wounds are characterized by oxygen levels lower than that of healthy skin (hypoxia) and poor healing. To better understand the pathophysiology of impaired wound healing, we investigated how switching from high (21%) to low (2%) oxygen levels directly affects cultured skin myofibroblasts, essential cells for the normal wound repair process. Myofibroblast differentiation and function were assessed by quantifying α-smooth muscle actin expression and cell contraction in collagen gels and on wrinkling silicone substrates. Culture for 5 days at 2% oxygen is perceived as hypoxia and significantly reduced myofibroblast differentiation and contraction despite high levels of the profibrotic transforming growth factor-β1. Analysis of α-smooth muscle actin expression on wrinkling substrates over time showed that reduced myofibroblast contraction preceded α-smooth muscle actin disassembly from stress fibers after switching from 21 to 2% oxygen. These effects were reversible by restoring high oxygen conditions and by applying mechanical stress. We suggest that mechanical challenge is a clinical relevant strategy to improve ischemic and chronic wound healing by supporting myofibroblast formation.

Keywords
Animals, Cell Differentiation/physiology, Cell Division/physiology, Cells, Cultured, Fibroblasts/cytology, Fibroblasts/metabolism, Humans, Hypoxia/metabolism, Hypoxia/pathology, Hypoxia/physiopathology, Ischemia/metabolism, Ischemia/pathology, Ischemia/physiopathology, Oxygen/metabolism, Oxygen/pharmacology, Rats, Skin/injuries, Skin/metabolism, Skin/pathology, Subcutaneous Tissue/physiology, Wound Healing/physiology, Wounds and Injuries/metabolism, Wounds and Injuries/pathology, Wounds and Injuries/physiopathology
Pubmed
Web of science
Open Access
Yes
Create date
16/01/2018 15:08
Last modification date
20/08/2019 15:01
Usage data