One hundred base pairs of 5' flanking sequence of a vaccinia virus late gene are sufficient to temporally regulate late transcription.

Details

Serval ID
serval:BIB_99DF3377644F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
One hundred base pairs of 5' flanking sequence of a vaccinia virus late gene are sufficient to temporally regulate late transcription.
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Bertholet C., Drillien R., Wittek R.
ISSN
0027-8424[print], 0027-8424[linking]
Publication state
Published
Issued date
04/1985
Volume
82
Number
7
Pages
2096-2100
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
A vaccinia virus late gene coding for a major structural polypeptide of 11 kDa was sequenced. Although the 5' flanking gene region is very A+T rich, it shows little homology either to the corresponding region of vaccinia early genes or to consensus sequences characteristic of most eukaryotic genes. Three DNA fragments (100, 200, and 500 base pairs, respectively), derived from the flanking region and including the late gene mRNA start site, were inserted into the coding sequence of the vaccinia virus thymidine kinase (TK) early gene by homologous in vivo recombination. Recombinants were selected on the basis of their TK- phenotype. Cells were infected with the recombinant viruses and RNA was isolated at 1-hr intervals. Transcripts initiating either from the TK early promoter, or from the late gene promoter at its authentic position, or from the translocated late gene promoters within the early gene were detected by nuclease S1 mapping. Early after infection, only transcripts from the TK early promoter were detected. Later in infection, however, transcripts were also initiated from the translocated late promoters. This RNA appeared at the same time and in similar quantities as the RNA from the late promoter at its authentic position. No quantitative differences in promoter efficiency between the 100-, 200-, and 500-base-pair insertions were observed. We conclude that all necessary signals for correct regulation of late-gene expression reside within only 100 base pairs of 5' flanking sequence.
Keywords
Amino Acid Sequence, Animals, Base Composition, Base Sequence, Chick Embryo, DNA, Recombinant, Genes, Viral, RNA, Messenger/analysis, Thymidine Kinase/genetics, Transcription, Genetic, Translocation, Genetic, Vaccinia virus/genetics, Viral Proteins/genetics, Viral Structural Proteins
Pubmed
Web of science
Create date
24/01/2008 10:43
Last modification date
20/08/2019 15:01
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