Radioimmunoassay versus flow cytometric assay to quantify LPS-binding protein (LBP) concentrations in human plasma.

Details

Serval ID
serval:BIB_99DB066FC3CA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Radioimmunoassay versus flow cytometric assay to quantify LPS-binding protein (LBP) concentrations in human plasma.
Journal
Journal of immunological methods
Author(s)
Heumann D., Gallay P., Le Roy D., Landmann R., Glauser M.P.
ISSN
0022-1759 (Print)
ISSN-L
0022-1759
Publication state
Published
Issued date
16/05/1994
Peer-reviewed
Oui
Volume
171
Number
2
Pages
169-176
Language
english
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
LPS-binding protein (LBP) is an acute phase protein present in plasma, that has been shown to play a major role in sensitizing monocytes to LPS. We describe here two assays to quantify LBP in plasma. The first assay made use of monophosphoryl lipid A to capture LBP in human plasma, and LBP was detected by radiolabeled anti-LBP IgG. The second assay measured LBP by flow cytometry, using LBPs ability to present LPS to the CD14 receptor present on monocytes. Both assays had a similar level of sensitivity, that allowed the quantification of LBP in human plasma.
Keywords
Acute-Phase Proteins/analysis, Antibody Specificity, Carrier Proteins/blood, Flow Cytometry, Humans, Immunoglobulin G, Lipid A/analogs & derivatives, Membrane Glycoproteins, Radioimmunoassay, Sensitivity and Specificity
Pubmed
Web of science
Create date
06/08/2018 10:33
Last modification date
20/08/2019 15:01
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