IFT proteins interact with HSET to promote supernumerary centrosome clustering in mitosis.

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Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_9991C1CE2399
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
IFT proteins interact with HSET to promote supernumerary centrosome clustering in mitosis.
Journal
EMBO reports
Author(s)
Vitre B., Taulet N., Guesdon A., Douanier A., Dosdane A., Cisneros M., Maurin J., Hettinger S., Anguille C., Taschner M., Lorentzen E., Delaval B.
ISSN
1469-3178 (Electronic)
ISSN-L
1469-221X
Publication state
Published
Issued date
04/06/2020
Peer-reviewed
Oui
Volume
21
Number
6
Pages
e49234
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Centrosome amplification is a hallmark of cancer, and centrosome clustering is essential for cancer cell survival. The mitotic kinesin HSET is an essential contributor to this process. Recent studies have highlighted novel functions for intraflagellar transport (IFT) proteins in regulating motors and mitotic processes. Here, using siRNA knock-down of various IFT proteins or AID-inducible degradation of endogenous IFT88 in combination with small-molecule inhibition of HSET, we show that IFT proteins together with HSET are required for efficient centrosome clustering. We identify a direct interaction between the kinesin HSET and IFT proteins, and we define how IFT proteins contribute to clustering dynamics during mitosis using high-resolution live imaging of centrosomes. Finally, we demonstrate the requirement of IFT88 for efficient centrosome clustering in a variety of cancer cell lines naturally harboring supernumerary centrosomes and its importance for cancer cell proliferation. Overall, our data unravel a novel role for the IFT machinery in centrosome clustering during mitosis in cells harboring supernumerary centrosomes.
Keywords
HSET/KifC1, IFT proteins, cancer, centrosome clustering, mitosis
Pubmed
Web of science
Open Access
Yes
Create date
09/07/2020 21:28
Last modification date
30/04/2021 6:13
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