Immunocytochemical localization of opsin in outer segments and Golgi zones of frog photoreceptor cells. An electron microscope analysis of cross-linked albumin-embedded retinas

Details

Serval ID
serval:BIB_9987AF64B08D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Immunocytochemical localization of opsin in outer segments and Golgi zones of frog photoreceptor cells. An electron microscope analysis of cross-linked albumin-embedded retinas
Journal
Journal of Cell Biology
Author(s)
Papermaster  D. S., Schneider  B. G., Zorn  M. A., Kraehenbuhl  J. P.
ISSN
0021-9525 (Print)
Publication state
Published
Issued date
04/1978
Volume
77
Number
1
Pages
196-210
Notes
Journal Article --- Old month value: Apr
Abstract
Adult vertebrate retinal cells (rod and cones) continuously synthesize membrane proteins and transport them to the organelle specialized for photon capture, the outer segment. The cell structures involved in the synthesis of opsin have been identified by means of immunocytochemistry at the electron microscope level. Two indirect detection systems were used: (a) rabbit antibodies to frog opsin were localized with ferritin conjugated F(ab')2 of sheep antibodies to rabbit F(ab')2 and (b) sheep antibodies to cattle opsin were coupled to biotin and visualized by means of avidin-ferritin conjugates (AvF). The reagents were applied directly to the surface of thin sections of frog retinal tissues embedded in glutaraldehyde cross-linked bovine serum albumin (BSA). Specific binding of anti-opsin antibodies indicates that opsin is localized in the disks of rod outer segments (ROS), as expected, and in the Golgi zone of the rod cell inner segments. In addition, we observed quantitatively different labeling patterns of outer segments of rods and cones with each of the sera employed. These reactions may indicate immunological homology of rod and cone photopigments. Because these quantitiative variations of labeling density extend along the entire length of the outer segment, they also serve to identify the cell which has shed its disks into adjacent pigment ipithelial cell phagosomes.
Keywords
Animals Anura Cell Membrane/analysis Cytoplasm/analysis Eye Proteins/*analysis Fluorescent Antibody Technique Golgi Apparatus/*analysis Organoids/analysis Photoreceptors/*analysis/ultrastructure Rana pipiens Retina/ultrastructure Retinal Pigments/*analysis
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 15:06
Last modification date
20/08/2019 15:01
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