T cell receptor alpha gene rearrangement and transcription in adult thymic gamma delta cells.

Details

Serval ID
serval:BIB_983
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
T cell receptor alpha gene rearrangement and transcription in adult thymic gamma delta cells.
Journal
European Journal of Immunology
Author(s)
Mertsching E., Wilson A., MacDonald H.R., Ceredig R.
ISSN
0014-2980
Publication state
Published
Issued date
1997
Peer-reviewed
Oui
Volume
27
Number
2
Pages
389-396
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Abstract
T cells belong to two separate lineages based on surface expression of alpha beta or gamma delta T cell receptors (TCR). Since during thymus development TCR beta, gamma, and delta genes rearrange before alpha genes, and gamma delta cells appear earlier than alpha beta cells, it has been assumed that gamma delta cells are devoid of TCR alpha rearrangements. We show here that this is not the case, since mature adult, but not fetal, thymic gamma delta cells undergo VJ alpha rearrangements more frequently than immature alpha beta lineage thymic precursors. Sequence analysis shows VJ alpha rearrangements in gamma delta cells to be mostly (70%) nonproductive. Furthermore, VJ alpha rearrangements in gamma delta cells are transcribed normally and, as shown by analysis of TCR beta-/- mice, occur independently of productive VDJ beta rearrangements. These data are interpreted in the context of a model in which precursors of alpha beta and gamma delta cells differ in their ability to express a functional pre-TCR complex.
Keywords
Animals, Cell Differentiation/genetics, Cell Differentiation/immunology, Female, Gene Rearrangement, T-Lymphocyte/genetics, Male, Mice, Mice, Inbred C57BL, Mice, Inbred DBA, Receptors, Antigen, T-Cell, alpha-beta/genetics, Receptors, Antigen, T-Cell, gamma-delta/analysis, T-Lymphocyte Subsets/immunology, Thymus Gland/cytology, Thymus Gland/immunology, Transcription, Genetic/immunology
Pubmed
Web of science
Create date
19/11/2007 12:48
Last modification date
20/08/2019 14:59
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