Antigen-induced airway hyperresponsiveness, pulmonary eosinophilia, and chemokine expression in B cell-deficient mice

Details

Serval ID
serval:BIB_982703FC121D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Antigen-induced airway hyperresponsiveness, pulmonary eosinophilia, and chemokine expression in B cell-deficient mice
Journal
American Journal of Respiratory Cell and Molecular Biology
Author(s)
MacLean  J. A., Sauty  A., Luster  A. D., Drazen  J. M., De Sanctis  G. T.
ISSN
1044-1549 (Print)
Publication state
Published
Issued date
03/1999
Volume
20
Number
3
Pages
379-87
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Mar
Abstract
Murine models of allergen-induced pulmonary inflammation share many features with human asthma, including the development of antigen-induced pulmonary eosinophilia, airway hyperresponsiveness, antigen-specific cellular and antibody responses, the elaboration of Th2 cytokines (interleukin [IL]-4 and IL-5), and the expression of chemokines with activity for eosinophils. We examined the role of B cells and antigen-specific antibody responses in such a model by studying the histopathologic and physiologic responses of B cell-deficient mice compared with wild-type controls, following systemic immunization and airway challenge with ovalbumin (OVA). Both OVA-challenged wild-type and B cell-deficient mice developed (1) airway hyperresponsiveness, (2) pulmonary inflammation with activated T cells and eosinophils, (3) IL-4 and IL-5 secretion into the airway lumen, and (4) increased expression of the eosinophil active chemokines eotaxin and monocyte chemotactic protein-3. There were no significant differences in either the pathologic or physiologic responses in the B cell-deficient mice compared with wild-type mice. These data indicate that B cells and antigen-specific antibodies are not required for the development of airway hyperresponsiveness, eosinophilic pulmonary inflammation, and chemokine expression in sensitized mice following aerosol challenge with antigen.
Keywords
Animals Antibody Specificity Asthma/immunology B-Lymphocytes/*immunology Bronchoalveolar Lavage Fluid/cytology/immunology Chemokines/*analysis/genetics Immunoglobulin E/immunology Lung/pathology Mice Mice, Mutant Strains Ovalbumin/immunology Pulmonary Eosinophilia/*immunology RNA, Messenger/analysis Respiratory Hypersensitivity/*immunology
Pubmed
Web of science
Create date
25/01/2008 9:52
Last modification date
20/08/2019 14:59
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