Hydrogel-based milliwell arrays for standardized and scalable retinal organoid cultures.

Details

Serval ID
serval:BIB_981417E59C16
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Hydrogel-based milliwell arrays for standardized and scalable retinal organoid cultures.
Journal
Scientific reports
Author(s)
Decembrini S., Hoehnel S., Brandenberg N., Arsenijevic Y., Lutolf M.P.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
24/06/2020
Peer-reviewed
Oui
Volume
10
Number
1
Pages
10275
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
The development of improved methods to culture retinal organoids is relevant for the investigation of mechanisms of retinal development under pathophysiological conditions, for screening of neuroprotective compounds, and for providing a cellular source for clinical transplantation. We report a tissue-engineering approach to accelerate and standardize the production of retinal organoids by culturing mouse embryonic stem cells (mESC) in optimal physico-chemical microenvironments. Arrayed round-bottom milliwells composed of biomimetic hydrogels, combined with an optimized medium formulation, promoted the rapid generation of retina-like tissue from mESC aggregates in a highly efficient and stereotypical manner: ∼93% of the aggregates contained retinal organoid structures. 26 day-old retinal organoids were composed of ∼80% of photoreceptors, of which ∼22% are GNAT2-positive cones, an important and rare sensory cell type that is difficult to study in rodent models. The compartmentalization of retinal organoids into predefined locations on a two-dimensional array not only allowed us to derive almost all aggregates into retinal organoids, but also to reliably capture the dynamics of individual organoids, an advantageous requirement for high-throughput experimentation. Our improved retinal organoid culture system should be useful for applications that require scalability and single-organoid traceability.
Pubmed
Open Access
Yes
Create date
03/07/2020 17:28
Last modification date
16/07/2020 8:43
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