Assessment of the clinical significance of antigenic and functional levels of α1-proteinase inhibitor (α1-Pi) in infiltrating ductal breast carcinomas.
Details
Serval ID
serval:BIB_97E86D21D646
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Assessment of the clinical significance of antigenic and functional levels of α1-proteinase inhibitor (α1-Pi) in infiltrating ductal breast carcinomas.
Journal
Clinical biochemistry
ISSN
1873-2933 (Electronic)
ISSN-L
0009-9120
Publication state
Published
Issued date
11/2012
Peer-reviewed
Oui
Volume
45
Number
16-17
Pages
1421-1431
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
To determine the clinical significance of α1-proteinase inhibitor (α1-Pi) in infiltrating ductal breast carcinoma patients.
Serum levels of α1-Pi, tryptic specific inhibitory capacity and α1-Pi circulating immune complexes were determined using radial immunodiffusion, BAPNA assays and ELISA, respectively. 2-DE-MS and immunohistochemistry were performed to examine α1-Pi protein expression.
A decreased serum level of α1-Pi was found among breast cancer patients in comparison to controls. In addition, we found a significantly decreased mean level of α1-Pi in the node metastatic group when compared to node negative patients. However, the functional activity of the inhibitor did not decrease proportionately. Through 2-DE analyses, a differential expression of α1-Pi isoforms according to tumor stage and node metastatic development was found.
Both α1-Pi levels and specific activity could be a source of complementary clinical information and may provide useful information for a better understanding of the mechanisms of metastasis.
Serum levels of α1-Pi, tryptic specific inhibitory capacity and α1-Pi circulating immune complexes were determined using radial immunodiffusion, BAPNA assays and ELISA, respectively. 2-DE-MS and immunohistochemistry were performed to examine α1-Pi protein expression.
A decreased serum level of α1-Pi was found among breast cancer patients in comparison to controls. In addition, we found a significantly decreased mean level of α1-Pi in the node metastatic group when compared to node negative patients. However, the functional activity of the inhibitor did not decrease proportionately. Through 2-DE analyses, a differential expression of α1-Pi isoforms according to tumor stage and node metastatic development was found.
Both α1-Pi levels and specific activity could be a source of complementary clinical information and may provide useful information for a better understanding of the mechanisms of metastasis.
Keywords
Adult, Amino Acid Sequence, Antigens, Neoplasm/blood, Antigens, Neoplasm/immunology, Area Under Curve, Biomarkers, Tumor/blood, Biomarkers, Tumor/chemistry, Biomarkers, Tumor/immunology, Biomarkers, Tumor/metabolism, Breast Neoplasms/blood, Breast Neoplasms/immunology, Breast Neoplasms/pathology, Carcinoma, Ductal, Breast/blood, Carcinoma, Ductal, Breast/immunology, Carcinoma, Ductal, Breast/secondary, Case-Control Studies, Female, Humans, Lymphatic Metastasis, Middle Aged, Molecular Sequence Data, Peptide Fragments/chemistry, Protein Isoforms/blood, Protein Isoforms/immunology, Proteolysis, ROC Curve, Trypsin/chemistry, Trypsin Inhibitors/blood, Trypsin Inhibitors/immunology, alpha 1-Antitrypsin/blood, alpha 1-Antitrypsin/chemistry, alpha 1-Antitrypsin/immunology, alpha 1-Antitrypsin/metabolism
Pubmed
Web of science
Create date
17/10/2023 8:18
Last modification date
20/10/2023 6:10
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