A single-amino-acid substitution in polyomavirus VP1 correlates with plaque size and hemagglutination behavior

Details

Serval ID
serval:BIB_97C387F4EC86
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A single-amino-acid substitution in polyomavirus VP1 correlates with plaque size and hemagglutination behavior
Journal
Journal of Virology
Author(s)
Freund  R., Garcea  R. L., Sahli  R., Benjamin  T. L.
ISSN
0022-538X (Print)
Publication state
Published
Issued date
01/1991
Volume
65
Number
1
Pages
350-5
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jan
Abstract
The plaque size and hemagglutination characteristics of five cloned wild-type strains of polyomavirus were determined. The strains fell into two groups, those with large or small plaques, each with distinctive hemagglutination behavior at different temperatures and pHs. The nucleotide sequence of VP1, the major capsid protein of the virus, was determined for each of the viral strains. The PTA (large-plaque) and RA (small-plaque) strains differed only at residue 92 of VP1, where there is a glutamic acid or glycine, respectively (R. Freund, A. Calderone, C. J. Dawe, and T. L. Benjamin, J. Virol. 65:335-341, 1991). The same amino acid difference in VP1 correlated with plaque size and hemagglutination properties of the other sequenced viruses. Mutagenesis converting amino acid 92 from glutamic acid to glycine converted the plaque size and hemagglutination behavior of the large-plaque PTA strain to that of a small-plaque strain. Furthermore, PTA and RA VP1 proteins produced in Escherichia coli behaved as their parental viruses did in hemagglutination assays. These results demonstrate that amino acid residue 92 of VP1 is involved in determining the plaque size and hemagglutination behavior of polyomavirus and strongly suggest that this region of the VP1 polypeptide interacts directly with cell receptors.
Keywords
Amino Acid Sequence Animals Capsid/*genetics/isolation & purification Capsid Proteins Cloning, Molecular Erythrocytes/immunology *Hemagglutination, Viral Hydrogen-Ion Concentration Kinetics Mice Molecular Sequence Data *Mutagenesis, Site-Directed Plaque Assay Polyomavirus/genetics/*physiology Recombinant Proteins/isolation & purification Sheep Species Specificity
Pubmed
Web of science
Create date
24/01/2008 20:51
Last modification date
20/08/2019 14:59
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