Glycosylphosphatidylinositol-anchored glucanosyltransferases play an active role in the biosynthesis of the fungal cell wall.

Details

Serval ID
serval:BIB_97C33F3A42DF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Glycosylphosphatidylinositol-anchored glucanosyltransferases play an active role in the biosynthesis of the fungal cell wall.
Journal
Journal of Biological Chemistry
Author(s)
Mouyna I., Fontaine T., Vai M., Monod M., Fonzi W.A., Diaquin M., Popolo L., Hartland R.P., Latgé J.P.
ISSN
0021-9258
Publication state
Published
Issued date
05/2000
Peer-reviewed
Oui
Volume
275
Number
20
Pages
14882-14889
Language
english
Abstract
A novel 1,3-beta-glucanosyltransferase isolated from the cell wall of Aspergillus fumigatus was recently characterized. This enzyme splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end to the non-reducing end of another 1, 3-beta-glucan molecule forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains. The GEL1 gene encoding this enzyme was cloned and sequenced. The predicted amino acid sequence of Gel1p was homologous to several yeast protein families encoded by GAS of Saccharomyces cerevisiae, PHR of Candida albicans, and EPD of Candida maltosa. Although the expression of these genes is required for correct morphogenesis in yeast, the biochemical function of the encoded proteins was unknown. The biochemical assays performed on purified recombinant Gas1p, Phr1p, and Phr2p showed that these proteins have a 1,3-beta-glucanosyltransferase activity similar to that of Gel1p. Biochemical data and sequence analysis have shown that Gel1p is attached to the membrane through a glycosylphosphatidylinositol in a similar manner as the yeast homologous proteins. The activity has been also detected in membrane preparations, showing that this 1,3-beta-glucanosyltransferase is indeed active in vivo. Our results show that transglycosidases anchored to the plasma membrane via glycosylphosphatidylinositols can play an active role in fungal cell wall synthesis.
Keywords
Amino Acid Sequence, Aspergillus fumigatus/genetics, Aspergillus fumigatus/metabolism, Cell Wall/metabolism, Chitin/biosynthesis, Cloning, Molecular, Conserved Sequence, Genes, Fungal, Glucan Endo-1,3-beta-D-Glucosidase/chemistry, Glucan Endo-1,3-beta-D-Glucosidase/genetics, Glucans/biosynthesis, Glycosylphosphatidylinositols/metabolism, Molecular Sequence Data, Recombinant Proteins/chemistry, Recombinant Proteins/metabolism, Sequence Alignment, Sequence Homology, Amino Acid
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 16:46
Last modification date
20/08/2019 14:59
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