Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis
Details
Serval ID
serval:BIB_97B6765E6192
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis
Journal
Journal of Clinical Microbiology
ISSN
0095-1137 (Print)
Publication state
Published
Issued date
02/1993
Volume
31
Number
2
Pages
175-8
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb
Research Support, Non-U.S. Gov't --- Old month value: Feb
Abstract
A method for the rapid identification of mycobacteria to the species level was developed on the basis of evaluation by the polymerase chain reaction (PCR) of the gene encoding for the 65-kDa protein. The method involves restriction enzyme analysis of PCR products obtained with primers common to all mycobacteria. Using two restriction enzymes, BstEII and HaeIII, medically relevant and other frequent laboratory isolates were differentiated to the species or subspecies level by PCR-restriction enzyme pattern analysis. PCR-restriction enzyme pattern analysis was performed on isolates (n = 330) from solid and fluid culture media, including BACTEC, or from frozen and lyophilized stocks. The procedure does not involve hybridization steps or the use of radioactivity and can be completed within 1 working day.
Keywords
Bacterial Proteins/genetics
*Bacterial Typing Techniques
Base Sequence
DNA Restriction Enzymes
DNA, Bacterial/genetics
Evaluation Studies
Genes, Bacterial
Heat-Shock Proteins/genetics
Humans
Molecular Sequence Data
Mycobacterium/*classification/*genetics/isolation & purification
Mycobacterium Infections/diagnosis
Polymerase Chain Reaction/*methods
Species Specificity
Pubmed
Web of science
Create date
25/01/2008 15:46
Last modification date
20/08/2019 15:59