Recombinant antigens as diagnostic markers for aspergillosis

Details

Serval ID
serval:BIB_97615A2DD387
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Recombinant antigens as diagnostic markers for aspergillosis
Journal
Diagnostic Microbiology and Infectious Disease
Author(s)
Sarfati  J., Monod  M., Recco  P., Sulahian  A., Pinel  C., Candolfi  E., Fontaine  T., Debeaupuis  J. P., Tabouret  M., Latge  J. P.
ISSN
0732-8893 (Print)
Publication state
Published
Issued date
08/2006
Volume
55
Number
4
Pages
279-91
Notes
Journal Article --- Old month value: Aug
Abstract
Eight recombinant proteins and purified galactomannan of Aspergillus fumigatus were tested by enzyme-linked immunosorbent assay to quantify the anti-Aspergillus antibodies in sera of patients with aspergilloma, allergic bronchopulmonary aspergillosis (ABPA), and invasive aspergillosis (IA). In spite of the variability observed in the immune responses of individual patients, quantification of the antibody titers against the 18-kDa ribonuclease (RNU), the 360-kDa catalase (CAT), and the 88-kDa dipeptidylpeptidase V (DPPV) was useful for the diagnosis of aspergilloma and ABPA. Differential diagnosis of ABPA was even possible among cystic fibrosis as well as noncystic fibrosis patients. In the group of immunocompromised patients with IA, no antibody response was mounted in response to the Aspergillus infection in any of the patients. Interestingly, about half of the patients with proven IA came to the hospital with high titers of anti-Aspergillus antibodies, suggesting that they were infected upon entry to the hospital. These results suggest that recombinant RNU, CAT, and DPPV have a great potential in the serodiagnosis of all forms of aspergillosis in the immunocompromised and immunocompetent patient.
Keywords
Antigens, Bacterial/*biosynthesis/immunology Aspergillosis/*diagnosis/immunology Aspergillus fumigatus/genetics/*immunology Cystic Fibrosis/immunology/microbiology Enzyme-Linked Immunosorbent Assay/methods Genetic Vectors/biosynthesis Humans Predictive Value of Tests Recombinant Proteins/immunology
Pubmed
Web of science
Create date
25/01/2008 16:46
Last modification date
20/08/2019 14:59
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