The promoter of the gene encoding 3',5'-cyclic adenosine monophosphate (cAMP) response element binding protein contains cAMP response elements: evidence for positive autoregulation of gene transcription.

Details

Serval ID
serval:BIB_972AF8F1FFFE
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The promoter of the gene encoding 3',5'-cyclic adenosine monophosphate (cAMP) response element binding protein contains cAMP response elements: evidence for positive autoregulation of gene transcription.
Journal
Endocrinology
Author(s)
Meyer T.E., Waeber G., Lin J., Beckmann W., Habener J.F.
ISSN
0013-7227
Publication state
Published
Issued date
1993
Peer-reviewed
Oui
Volume
132
Number
2
Pages
770-80
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S. - Publication Status: ppublish
Abstract
The transcriptional transactivational activities of the phosphoprotein cAMP-response element-binding protein (CREB) are activated by the cAMP-dependent protein kinase A signaling pathway. Dimers of CREB bind to the palindromic DNA element 5'-TGACGTCA-3' (or similar motifs) called cAMP-responsive enhancers (CREs) found in the control regions of many genes, and activate transcription in response to phosphorylation of CREB by protein kinase A. Earlier we reported on the cyclical expression of the CREB gene in the Sertoli cells of the rat testis that occurred concomitant with the FSH-induced rise in cellular cAMP levels and suggested that transcription of the CREB gene may be autoregulated by cAMP-dependent transcriptional proteins. We now report the structure of the 5'-flanking sequence of the human CREB gene containing promoter activity. The promoter has a high content of guanosines and cytosines and lacks canonical TATA and CCAAT boxes typically found in the promoters of genes in eukaryotes. Notably, the promoter contains three CREs and transcriptional activities of a promoter-luciferase reporter plasmid transfected to placental JEG-3 cells are increased 3- to 5-fold over basal activities in response to either cAMP or 12-O-tetradecanoyl phorbol-14-acetate, and give 6- to 7-fold responses when both agents are added. The CREs bind recombinant CREB and endogenous CREB or CREB-like proteins contained in placental JEG-3 cells and also confer cAMP-inducible transcriptional activation to a heterologous minimal promoter. Our studies suggest that the expression of the CREB gene is positively autoregulated in trans.
Keywords
Amino Acid Sequence, Base Sequence, Cell Nucleus, Choriocarcinoma, Cloning, Molecular, Cyclic AMP, Cyclic AMP Response Element-Binding Protein, DNA, Exons, Female, Gene Expression Regulation, Neoplastic, Humans, Luciferases, Molecular Sequence Data, Nuclear Proteins, Oligodeoxyribonucleotides, Pregnancy, Promoter Regions, Genetic, Protein Biosynthesis, Protein Kinases, RNA, Neoplasm, Recombinant Fusion Proteins, Transcription, Genetic, Tumor Cells, Cultured, Uterine Neoplasms
Pubmed
Web of science
Create date
25/01/2008 14:10
Last modification date
20/08/2019 14:59
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