Uncoupling phosphate deficiency from its major effects on growth and transcriptome via PHO1 expression in Arabidopsis.

Details

Serval ID
serval:BIB_96E6532AB970
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Uncoupling phosphate deficiency from its major effects on growth and transcriptome via PHO1 expression in Arabidopsis.
Journal
The Plant journal
Author(s)
Rouached H., Stefanovic A., Secco D., Bulak Arpat A., Gout E., Bligny R., Poirier Y.
ISSN
1365-313X (Electronic)
ISSN-L
0960-7412
Publication state
Published
Issued date
02/2011
Peer-reviewed
Oui
Volume
65
Number
4
Pages
557-570
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Inorganic phosphate (Pi) is one of the most limiting nutrients for plant growth in both natural and agricultural contexts. Pi-deficiency leads to a strong decrease in shoot growth, and triggers extensive changes at the developmental, biochemical and gene expression levels that are presumably aimed at improving the acquisition of this nutrient and sustaining growth. The Arabidopsis thaliana PHO1 gene has previously been shown to participate in the transport of Pi from roots to shoots, and the null pho1 mutant has all the hallmarks associated with shoot Pi deficiency. We show here that A. thaliana plants with a reduced expression of PHO1 in roots have shoot growth similar to Pi-sufficient plants, despite leaves being strongly Pi deficient. Furthermore, the gene expression profile normally triggered by Pi deficiency is suppressed in plants with low PHO1 expression. At comparable levels of shoot Pi supply, the wild type reduces shoot growth but maintains adequate shoot vacuolar Pi content, whereas the PHO1 underexpressor maintains maximal growth with strongly depleted Pi reserves. Expression of the Oryza sativa (rice) PHO1 ortholog in the pho1 null mutant also leads to plants that maintain normal growth and suppression of the Pi-deficiency response, despite the low shoot Pi. These data show that it is possible to unlink low shoot Pi content with the responses normally associated with Pi deficiency through the modulation of PHO1 expression or activity. These data also show that reduced shoot growth is not a direct consequence of Pi deficiency, but is more likely to be a result of extensive gene expression reprogramming triggered by Pi deficiency.

Keywords
Arabidopsis/genetics, Arabidopsis/growth & development, Arabidopsis/metabolism, Arabidopsis Proteins/genetics, Arabidopsis Proteins/metabolism, Gene Expression Profiling, Gene Expression Regulation, Plant, Mutation, Oligonucleotide Array Sequence Analysis, Phosphates/metabolism, Plant Roots/metabolism, Plant Shoots/growth & development, Plants, Genetically Modified/genetics, Plants, Genetically Modified/growth & development, Plants, Genetically Modified/metabolism, RNA, Plant/genetics
Pubmed
Web of science
Create date
16/02/2012 14:58
Last modification date
20/08/2019 14:58
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