Comparative gene finding in chicken indicates that we are closing in on the set of multi-exonic widely expressed human genes.

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Serval ID
serval:BIB_969A35C25E60
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Comparative gene finding in chicken indicates that we are closing in on the set of multi-exonic widely expressed human genes.
Journal
Nucleic Acids Research
Author(s)
Castelo R., Reymond A., Wyss C., Câmara F., Parra G., Antonarakis S.E., Guigó R., Eyras E.
ISSN
1362-4962[electronic], 0305-1048[linking]
Publication state
Published
Issued date
2005
Volume
33
Number
6
Pages
1935-1939
Language
english
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
The recent availability of the chicken genome sequence poses the question of whether there are human protein-coding genes conserved in chicken that are currently not included in the human gene catalog. Here, we show, using comparative gene finding followed by experimental verification of exon pairs by RT-PCR, that the addition to the multi-exonic subset of this catalog could be as little as 0.2%, suggesting that we may be closing in on the human gene set. Our protocol, however, has two shortcomings: (i) the bioinformatic screening of the predicted genes, applied to filter out false positives, cannot handle intronless genes; and (ii) the experimental verification could fail to identify expression at a specific developmental time. This highlights the importance of developing methods that could provide a reliable estimate of the number of these two types of genes.
Keywords
Animals, Chickens/genetics, Computational Biology/methods, Exons, Gene Expression, Genome, Human, Genomics/methods, Humans, Reverse Transcriptase Polymerase Chain Reaction
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 15:52
Last modification date
25/09/2019 6:10
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