Functional analysis of the 5' flanking sequence of a vaccinia virus late gene.

Details

Serval ID
serval:BIB_969555843F1E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Functional analysis of the 5' flanking sequence of a vaccinia virus late gene.
Journal
Embo Journal
Author(s)
Bertholet C., Stocco P., Van Meir E., Wittek R.
ISSN
0261-4189[print], 0261-4189[linking]
Publication state
Published
Issued date
08/1986
Volume
5
Number
8
Pages
1951-1957
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
A series of mutations, including 5' and 3' deletions, as well as insertions were introduced into the 5' flanking nucleotide sequence of a vaccinia virus late gene. This DNA has been shown previously to contain all the necessary elements for correct regulation of the gene most probably transcribed by the viral RNA polymerase. To facilitate the assays, the mutated DNA was fused to the chloramphenicol acetyltransferase gene and inserted into the genome of live vaccinia virus. The effects of the mutations on expression of the chimeric gene were studied by both enzyme assays and nuclease S1 analysis. The results showed that 5' deletions up to about 15 bp from the putative initiation site of transcription still yielded high levels of gene expression. All mutations, however, that deleted the authentic late mRNA start site, abolished promoter activity.
Keywords
Acetyltransferases/genetics, Animals, B-Lymphocytes/enzymology, Base Sequence, Cell Line, Cercopithecus aethiops, Chloramphenicol O-Acetyltransferase, DNA Restriction Enzymes, DNA, Recombinant/metabolism, DNA-Directed RNA Polymerases/metabolism, Genes, Genes, Bacterial, Genes, Regulator, Genes, Viral, Humans, Kidney, Plasmids, Thymidine Kinase/deficiency, Transcription, Genetic, Vaccinia virus/enzymology, Vaccinia virus/genetics
Pubmed
Web of science
Create date
24/01/2008 10:43
Last modification date
20/08/2019 14:58
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