Signaling steps involving the cytoplasmic domain of the interferon-gamma receptor alpha-subunit are not species-specific.

Details

Serval ID
serval:BIB_965771D73D73
Type
Article: article from journal or magazin.
Collection
Publications
Title
Signaling steps involving the cytoplasmic domain of the interferon-gamma receptor alpha-subunit are not species-specific.
Journal
Journal of Biological Chemistry
Author(s)
Böhni R., Hemmi S., Aguet M.
ISSN
0021-9258 (Print)
ISSN-L
0021-9258
Publication state
Published
Issued date
1994
Volume
269
Number
20
Pages
14541-14545
Language
english
Abstract
The ligand-binding chain of the interferon-gamma receptor (IFN-gamma R) is a unique cell surface protein which has no similarities to other cytokine receptors. Expression of this receptor chain (alpha-subunit) is not sufficient to mediate responsiveness to IFN-gamma. We and others have shown that IFN-gamma-mediated signal transduction requires a species-specific interaction of the extracellular portion of the known IFN-gamma receptor alpha-chain with an additional receptor subunit that was cloned recently and designated IFN-gamma R beta-chain or accessory factor 1. Here, we investigated whether this tight species barrier also applies to signaling events mediated by the cytoplasmic receptor domain. A cell line derived from embryos that lack the IFN-gamma R alpha-subunit was reconstituted with a hybrid mouse-human alpha-subunit that consisted of an extracellular murine and transmembrane and cytoplasmic human domains. The experiments reported herein showed that in mouse cells, the human intracellular domain of the hybrid IFN-gamma R alpha-subunit was fully functional and that, therefore, signaling steps involving this domain are not species-specific.
Keywords
Animals, Cells, Cultured, Chromosome Mapping, Chromosomes, Human, Pair 21, Cloning, Molecular, Cytoplasm/metabolism, Embryo, Mammalian, Fibroblasts/metabolism, Flow Cytometry, Humans, Interferon-gamma/pharmacology, Macromolecular Substances, Mice, Protein Multimerization, RNA, Messenger/biosynthesis, Receptors, Interferon/biosynthesis, Receptors, Interferon/genetics, Restriction Mapping, Signal Transduction
Pubmed
Web of science
Create date
28/01/2008 11:36
Last modification date
20/08/2019 14:58
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