The CbrA-CbrB two-component regulatory system controls the utilization of multiple carbon and nitrogen sources in Pseudomonas aeruginosa.

Details

Serval ID
serval:BIB_95E520B6BA91
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The CbrA-CbrB two-component regulatory system controls the utilization of multiple carbon and nitrogen sources in Pseudomonas aeruginosa.
Journal
Molecular microbiology
Author(s)
Nishijyo T., Haas D., Itoh Y.
ISSN
0950-382X
Publication state
Published
Issued date
2001
Peer-reviewed
Oui
Volume
40
Number
4
Pages
917-31
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Abstract
A novel two-component system, CbrA-CbrB, was discovered in Pseudomonas aeruginosa; cbrA and cbrB mutants of strain PAO were found to be unable to use several amino acids (such as arginine, histidine and proline), polyamines and agmatine as sole carbon and nitrogen sources. These mutants were also unable to use, or used poorly, many other carbon sources, including mannitol, glucose, pyruvate and citrate. A 7 kb EcoRI fragment carrying the cbrA and cbrB genes was cloned and sequenced. The cbrA and cbrB genes encode a sensor/histidine kinase (Mr 108 379, 983 residues) and a cognate response regulator (Mr 52 254, 478 residues) respectively. The amino-terminal half (490 residues) of CbrA appears to be a sensor membrane domain, as predicted by 12 possible transmembrane helices, whereas the carboxy-terminal part shares homology with the histidine kinases of the NtrB family. The CbrB response regulator shows similarity to the NtrC family members. Complementation and primer extension experiments indicated that cbrA and cbrB are transcribed from separate promoters. In cbrA or cbrB mutants, as well as in the allelic argR9901 and argR9902 mutants, the aot-argR operon was not induced by arginine, indicating an essential role for this two-component system in the expression of the ArgR-dependent catabolic pathways, including the aruCFGDB operon specifying the major aerobic arginine catabolic pathway. The histidine catabolic enzyme histidase was not expressed in cbrAB mutants, even in the presence of histidine. In contrast, proline dehydrogenase, responsible for proline utilization (Pru), was expressed in a cbrB mutant at a level comparable with that of the wild-type strain. When succinate or other C4-dicarboxylates were added to proline medium at 1 mM, the cbrB mutant was restored to a Pru+ phenotype. Such a succinate-dependent Pru+ property was almost abolished by 20 mM ammonia. In conclusion, the CbrA-CbrB system controls the expression of several catabolic pathways and, perhaps together with the NtrB-NtrC system, appears to ensure the intracellular carbon: nitrogen balance in P. aeruginosa.
Keywords
Amino Acid Sequence, Amino Acid Transport Systems, Bacterial Proteins, Base Sequence, Carbon, Carrier Proteins, Cloning, Molecular, Gene Expression Regulation, Bacterial, Histidine, Histidine Ammonia-Lyase, Molecular Sequence Data, Mutation, Nitrogen, Operon, Periplasmic Binding Proteins, Phosphoprotein Phosphatases, Protein Kinases, Pseudomonas aeruginosa, Repressor Proteins, Sequence Homology, Amino Acid, Transaminases, Transcription Factors
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 17:01
Last modification date
20/08/2019 14:58
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