Does exposure to inflammatory particles modify the pattern of anion in exhaled breath condensate?

Details

Ressource 1Download: Sauvain-J Breath Res 2020.pdf (798.72 [Ko])
State: Public
Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_95BADD049280
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Does exposure to inflammatory particles modify the pattern of anion in exhaled breath condensate?
Journal
Journal of breath research
Author(s)
Sauvain J.J., Edme J.L., Wild P., Suarez G., Bezerra OMPA, Talvani A., Algranti E., Carneiro APS, Chérot-Kornobis N., Sobaszek A., Hulo S.
ISSN
1752-7163 (Electronic)
ISSN-L
1752-7155
Publication state
Published
Issued date
14/02/2020
Peer-reviewed
Oui
Volume
14
Number
2
Pages
026005
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Exposure to environmental and occupational particulate matter (PM) induces health effects on the cardio-pulmonary system. In addition, associations between exposure to PM and metabolic syndromes like diabetes mellitus or obesity are now emerging in the literature. Collection of exhaled breath condensate (EBC) is an appealing non-invasive technique to sample pulmonary fluids. This hypothesis-generating study aims to (1) validate an ion chromatography method allowing the robust determination of different metabolism-related molecules (lactate, formate, acetate, propionate, butyrate, pyruvate, nitrite, nitrate) in EBC; (2) apply this method to EBC samples collected from workers exposed to quartz (a known inflammatory particle), to soapstone (a less inflammatory particle than quartz), as well as to controls. A multi-compound standard solution was used to determine the linearity range, detection limit, repeatability and bias from spiked EBC. The biological samples were injected without further treatment into an ion chromatograph with a conductivity detector. RTube <sup>®</sup> were used for field collection of EBC from 11 controls, 55 workers exposed to soapstone and 12 volunteers exposed to quartz dust. The analytical method used proved to be adequate for quantifying eight anions in EBC samples. Its sub-micromolar detection limits and repeatability, combined with a very simple sample preparation, allowed an easy and fast quantification of different glycolysis or nitrosative stress metabolites. Using multivariate discriminant analysis to maximize differences between groups, we observed a different pattern of anions with a higher formate/acetate ratio in the EBC samples for quartz exposed workers compared to the two other groups. We hypothesize that a modification of the metabolic signature could be induced by exposure to inflammatory particles like quartz and might be observed in the EBC via a change in the formate/acetate ratio.
Keywords
Exhaled breath condensate, ion chromatography, metabolism, quartz, small chain fatty acids, soapstone
Pubmed
Web of science
Open Access
Yes
Create date
04/12/2019 23:24
Last modification date
28/04/2020 7:09
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