Insulin inhibits tyrosinase activity and 5-S-cysteinyldopa formation in human melanoma cells.

Details

Serval ID
serval:BIB_95B3E9A7B8DE
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Insulin inhibits tyrosinase activity and 5-S-cysteinyldopa formation in human melanoma cells.
Journal
Acta Dermato-Venereologica
Author(s)
Benathan M., Labidi F.
ISSN
0001-5555
Publication state
Published
Issued date
07/1997
Peer-reviewed
Oui
Volume
77
Number
4
Pages
281-284
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Abstract
The effects of insulin on melanogenesis were examined in human Swift melanoma cells. When these cells were grown in a chemically defined culture medium containing insulin (5 microg/ml), they showed a low pigmentation in association with a high activity of glutathione peroxidase (GPO) and a low activity of tyrosinase. In Eagle's minimum essential medium supplemented with foetal calf serum (EMEM-FCS), the Swift cells showed an intense pigmentation in association with a low GPO activity and a high tyrosinase activity. Modulation of GPO activity with sodium selenite had no effect on melanogenesis variables. In contrast, addition of insulin (5 microg/ml) to the EMEM medium led to a marked decrease in tyrosinase activity (p<0.001) and to a concomitant reduction in the levels of 5-S-cysteinyldopa (p <0.01). These results indicate that insulin inhibits the formation of 5-S-cysteinyldopa and that of melanin via the inhibition of tyrosinase activity.
Keywords
Calcium, Chromatography, High Pressure Liquid, Cysteinyldopa, Glutathione Peroxidase, Glutathione Reductase, Humans, Insulin, Melanins, Melanoma, Monophenol Monooxygenase, Skin Neoplasms, Sodium Selenite, Tumor Cells, Cultured
Pubmed
Web of science
Create date
28/01/2008 9:20
Last modification date
20/08/2019 15:58
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