Automated microfluidic sorting of mammalian cells labeled with magnetic microparticles for those that efficiently express and secrete a protein of interest

Details

Serval ID
serval:BIB_951315D027DD
Type
Article: article from journal or magazin.
Collection
Publications
Title
Automated microfluidic sorting of mammalian cells labeled with magnetic microparticles for those that efficiently express and secrete a protein of interest
Journal
Biotechnology and Bioengineering
Author(s)
Droz X., Harraghy N., Lançon E., Le Fourn V., Calabrese D., Colombet T., Liechti P., Rida A., Girod P.A., Mermod N.
ISSN
1097-0290 (Electronic)
ISSN-L
0006-3592
Publication state
Published
Issued date
2017
Peer-reviewed
Oui
Volume
114
Number
8
Pages
1791-1802
Language
english
Abstract
We developed a method for the fast sorting and selection of mammalian cells expressing and secreting a protein at high levels. This procedure relies on cell capture using an automated microfluidic device handling antibody-coupled magnetic microparticles and on a timed release of the cells from the microparticles after capture. Using clinically compatible materials and procedures, we show that this approach is able to discriminate between cells that truly secrete high amounts of a protein from those that just display it at high levels on their surface without properly releasing it. When coupled to a cell colony imaging and picking device, this approach allowed the identification of CHO cell clones secreting a therapeutic protein at high levels that were not achievable without the cell sorting procedure.
Pubmed
Web of science
Create date
06/07/2017 17:50
Last modification date
20/08/2019 15:57
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