MAR elements regulate the probability of epigenetic switching between active and inactive gene expression.

Details

Serval ID
serval:BIB_9506ADE3A90F
Type
Article: article from journal or magazin.
Collection
Publications
Title
MAR elements regulate the probability of epigenetic switching between active and inactive gene expression.
Journal
Molecular BioSystems
Author(s)
Galbete J.L., Buceta M., Mermod N.
ISSN
1742-2051[electronic]
Publication state
Published
Issued date
2009
Volume
5
Number
2
Pages
143-150
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Abstract
Gene expression often cycles between active and inactive states in eukaryotes, yielding variable or noisy gene expression in the short-term, while slow epigenetic changes may lead to silencing or variegated expression. Understanding how cells control these effects will be of paramount importance to construct biological systems with predictable behaviours. Here we find that a human matrix attachment region (MAR) genetic element controls the stability and heritability of gene expression in cell populations. Mathematical modeling indicated that the MAR controls the probability of long-term transitions between active and inactive expression, thus reducing silencing effects and increasing the reactivation of silent genes. Single-cell short-terms assays revealed persistent expression and reduced expression noise in MAR-driven genes, while stochastic burst of expression occurred without this genetic element. The MAR thus confers a more deterministic behavior to an otherwise stochastic process, providing a means towards more reliable expression of engineered genetic systems.
Keywords
Animals, CHO Cells, Cricetinae, Cricetulus, Epigenesis, Genetic, Flow Cytometry, Gene Expression Regulation, Gene Silencing, Genetic Engineering, Humans, Matrix Attachment Regions/genetics, Models, Theoretical, Reverse Transcriptase Polymerase Chain Reaction, Time Factors, Transgenes
Pubmed
Web of science
Create date
23/03/2009 11:59
Last modification date
20/08/2019 15:57
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