Neonatal steroids induce a down-regulation of tenascin-C and elastin and cause a deceleration of the first phase and an acceleration of the second phase of lung alveolarization.

Details

Serval ID
serval:BIB_94E738FC8A6D
Type
Article: article from journal or magazin.
Collection
Publications
Title
Neonatal steroids induce a down-regulation of tenascin-C and elastin and cause a deceleration of the first phase and an acceleration of the second phase of lung alveolarization.
Journal
Histochemistry and Cell Biology
Author(s)
Roth-Kleiner M., Berger T.M., Gremlich S., Tschanz S.A., Mund S.I., Post M., Stampanoni M., Schittny J.C.
ISSN
1432-119X (Electronic)
ISSN-L
0948-6143
Publication state
Published
Issued date
2014
Volume
141
Number
1
Pages
75-84
Language
english
Notes
Publication types: Journal ArticlePublication Status: ppublish
Abstract
Pre- and postnatal corticosteroids are often used in perinatal medicine to improve pulmonary function in preterm infants. To mimic this clinical situation, newborn rats were treated systemically with dexamethasone (Dex), 0.1-0.01 mg/kg/day on days P1-P4. We hypothesized that postnatal Dex may have an impact on alveolarization by interfering with extracellular matrix proteins and cellular differentiation. Morphological alterations were observed on 3D images obtained by high-resolution synchrotron radiation X-ray tomographic microscopy. Alveolarization was quantified stereologically by estimating the formation of new septa between days P4 and P60. The parenchymal expression of tenascin-C (TNC), smooth muscle actin (SMA), and elastin was measured by immunofluorescence and gene expression for TNC by qRT-PCR. After Dex treatment, the first phase of alveolarization was significantly delayed between days P6 and P10, whereas the second phase was accelerated. Elastin and SMA expressions were delayed by Dex treatment, whereas TNC expression was delayed and prolonged. A short course of neonatal steroids impairs the first phase of alveolarization, most likely by altering the TNC and elastin expression. Due to an overshooting catch-up during the second phase of alveolarization, the differences disappear when the animals reach adulthood.
Pubmed
Web of science
Create date
07/02/2014 19:31
Last modification date
03/03/2018 19:38
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