Primary structure of an agonist binding subunit of the nicotinic acetylcholine receptor from bovine adrenal chromaffin cells

Details

Serval ID
serval:BIB_94D478828EEC
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Primary structure of an agonist binding subunit of the nicotinic acetylcholine receptor from bovine adrenal chromaffin cells
Journal
Neurochem Res
Author(s)
Criado  M., Alamo  L., Navarro  A.
ISSN
0364-3190
Publication state
Published
Issued date
03/1992
Peer-reviewed
Oui
Volume
17
Number
3
Pages
281-7
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar
Abstract
Activation by acetylcholine of a nicotinic acetylcholine receptor on the membrane of bovine chromaffin cells leads to membrane depolarization and to the subsequent triggering of catecholamine secretion. It is evident that acetylcholine receptors play a central role in the initial phase of the secretion process and, therefore, an extensive characterization of their molecular components and properties is of fundamental interest. With this intention, we have screened bovine adrenal medullary cDNA libraries with a probe coding for a fragment of the rat muscle acetylcholine receptor alpha subunit. Several cDNA clones were isolated. The longest cDNA had an open reading frame encoding a 495-amino acid protein with a molecular weight of 56,911. The deduced primary structure contains features that indicate that the encoded protein is an alpha or acetylcholine binding subunit, and, in fact, it manifests significant sequence similarity to previously cloned alpha subunits. Sequence identity is particularly high with the alpha 3 subunit, which is expressed in the rat pheochromocytoma PC12 cell line and in several brain areas, and, consequently, it is considered a component of a neuronal acetylcholine receptor. Accordingly, the present results suggest that the agonist binding subunit of the nicotinic acetylcholine receptor from bovine chromaffin cells is an alpha 3-type subunit, corroborating previous immunological and pharmacological evidence for the presence of a neuronal nicotinic receptor in chromaffin cells.
Keywords
Acetylcholine/*metabolism Adrenal Medulla/*chemistry/cytology Amino Acid Sequence Animals Base Sequence Cattle Cloning, Molecular DNA/chemistry Molecular Sequence Data Neurons/*chemistry Poly A/chemistry Receptors, Nicotinic/*chemistry Sequence Homology, Nucleic Acid
Pubmed
Web of science
Create date
08/04/2008 15:47
Last modification date
20/08/2019 14:57
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