Stereotactic coordinates for intracerebroventricular infusion after permanent focal cerebral ischemia in Wistar rats.

Details

Serval ID
serval:BIB_94B8B94CF577
Type
Article: article from journal or magazin.
Collection
Publications
Title
Stereotactic coordinates for intracerebroventricular infusion after permanent focal cerebral ischemia in Wistar rats.
Journal
Hippokratia
Author(s)
Lourbopoulos A., Chatzigeorgiou S., Mavridis T., Kokkinakis I., Tascos N., Simeonidou C.
ISSN
1108-4189 (Print)
ISSN-L
1108-4189
Publication state
Published
Issued date
01/2012
Peer-reviewed
Oui
Volume
16
Number
1
Pages
51-56
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Intracerebroventricular (ICV) experimental route is highly promising due to immediate approach of a "therapy" to the cerebrospinal compartment. Ischemic edema causes structural dislocations and stereotaxia alterations after temporary Middle Cerebral Artery Occlusion (t-MCAO), while there is no similar study for intracerebroventricular (ICV) invasion after permanent MCAO (p-MCAO).
Male Wistar rats were subjected to right p-MCAO and clinically evaluated 6 and 18 hours post-occlusion, using the modified Neurological Stroke Scale (mNSS) and modified Bederson's Scale (mBS). Infarction volume, hemispheric edema, middle line dislocation and stereotaxia of the lateral ventricles were studied at the same time-points.
P-MCAO induced mild but significant changes in the stereotaxia of the infarcted (ipsilateral) lateral ventricle on 18- (P<0.05), though not 6-hours (P>0.05) post-occlusion. These changes correlated with the mNSS and mBS scores (P<0.01) and allowed the expression of linear mathematical equations (stereotaxic coordinate = b0 + b1*mNSS; calculated by regression analysis) predicting the new ventricular position in each individual animal. The contralateral ventricular system was structurally unaffected on both time-points. Verification experiments indicated that the new coordinates were necessary on 18-hours post-occlusion for successful ICV invasion in all p-MCAO rats (Number Needed to Treat 2.28), compared to 56.25% success when using the classical coordinates for normal rats.
P-MCAO causes relatively late but predictable stereotaxia shifts for ICV invasion, which are different compared to t-MCAO.
Keywords
edema, intraventricular stereotaxic coordinates, lateral ventricles, permanent middle cerebral artery occlusion, stroke, transplantation
Pubmed
Web of science
Create date
18/03/2021 7:34
Last modification date
18/03/2021 7:34
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