The Anticancer Peptide TAT-RasGAP317-326 Exerts Broad Antimicrobial Activity.

Details

Ressource 1Download: fmicb-08-00994.pdf (2827.07 [Ko])
State: Public
Version: Final published version
Serval ID
serval:BIB_949E09781E9B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The Anticancer Peptide TAT-RasGAP317-326 Exerts Broad Antimicrobial Activity.
Journal
Frontiers in microbiology
Author(s)
Heulot M., Jacquier N., Aeby S., Le Roy D., Roger T., Trofimenko E., Barras D., Greub G., Widmann C.
ISSN
1664-302X (Print)
ISSN-L
1664-302X
Publication state
Published
Issued date
2017
Peer-reviewed
Oui
Volume
8
Pages
994
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Antibiotic resistance has become a major health issue. Nosocomial infections and the prevalence of resistant pathogenic bacterial strains are rising steadily. Therefore, there is an urgent need to develop new classes of antibiotics effective on multi-resistant nosocomial pathogenic bacteria. We have previously shown that a cell-permeable peptide derived from the p120 Ras GTPase-activating protein (RasGAP), called TAT-RasGAP317-326, induces cancer cell death, inhibits metastatic progression, and sensitizes tumor cells to various anti-cancer treatments in vitro and in vivo. We here report that TAT-RasGAP317-326 also possesses antimicrobial activity. In vitro, TAT-RasGAP317-326, but not mutated or truncated forms of the peptide, efficiently killed a series of bacteria including Escherichia coli, Acinetobacter baumannii, Staphylococcus aureus, and Pseudomonas aeruginosa. In vivo experiments revealed that TAT-RasGAP317-326 protects mice from lethal E. coli-induced peritonitis if administrated locally at the onset of infection. However, the protective effect was lost when treatment was delayed, likely due to rapid clearance and inadequate biodistribution of the peptide. Peptide modifications might overcome these shortcomings to increase the in vivo efficacy of the compound in the context of the currently limited antimicrobial options.

Keywords
RasGAP, TAT-RasGAP317−326, antimicrobial peptides, cell-permeable peptides
Pubmed
Web of science
Open Access
Yes
Create date
26/06/2017 17:38
Last modification date
20/08/2019 15:57
Usage data