High-throughput targeted screening in triple-negative breast cancer cells identifies Wnt-inhibiting activities in Pacific brittle stars.

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State: Public
Version: Final published version
Serval ID
serval:BIB_946ABACA1829
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
High-throughput targeted screening in triple-negative breast cancer cells identifies Wnt-inhibiting activities in Pacific brittle stars.
Journal
Scientific reports
Author(s)
Blagodatski A., Cherepanov V., Koval A., Kharlamenko V.I., Khotimchenko Y.S., Katanaev V.L.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
20/09/2017
Peer-reviewed
Oui
Volume
7
Number
1
Pages
11964
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Pro-proliferative oncogenic signaling is one of the hallmarks of cancer. Specific targeting of such signaling pathways is one of the main approaches to modern anti-cancer drug discovery, as opposed to more traditional search for general cytotoxic agents. Natural products, especially from marine sources, represent a largely untapped source of chemical diversity, which so far have mostly been screened for cytotoxicity. Here we present a pioneering pipeline of high-throughput screening of marine-based activities targeted against the Wnt signaling pathway, which is one of the key factors in oncogenic transformation, growth and metastasis in different cancers, including the devastating triple-negative breast cancer (TNBC) currently lacking any targeted therapies. This pipeline consisted of collection and characterization of numerous invertebrates during the SokhoBio expedition to the Kuril Basin in North Pacific, preparation of extracts from these specimen, and their screening in dedicated assays monitoring Wnt signaling in TNBC cells. This approach yielded a number of promising hits, including highly specific anti-Wnt activities targeting multiple levels within the Wnt pathway from Ophiura irrorata and other Pacific brittle stars.
Keywords
Animals, Antineoplastic Agents/isolation & purification, Antineoplastic Agents/pharmacology, Aquatic Organisms/chemistry, Biological Products/isolation & purification, Biological Products/pharmacology, Cell Line, Tumor, Drug Evaluation, Preclinical/methods, Echinodermata/chemistry, High-Throughput Screening Assays, Humans, Pacific Ocean, Triple Negative Breast Neoplasms/drug therapy, Wnt Proteins/antagonists & inhibitors, Wnt Signaling Pathway/drug effects
Pubmed
Web of science
Open Access
Yes
Create date
09/10/2017 10:35
Last modification date
17/09/2020 9:24
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