Comparison of five poxvirus genomes by analysis with restriction endonucleases HindIII, BamI and EcoRI.

Details

Serval ID
serval:BIB_93C4F6517D33
Type
Article: article from journal or magazin.
Collection
Publications
Title
Comparison of five poxvirus genomes by analysis with restriction endonucleases HindIII, BamI and EcoRI.
Journal
Journal of General Virology
Author(s)
Müller H.K., Wittek R., Schaffner W., Schümperli D., Menna A., Wyler R.
ISSN
0022-1317[print], 0022-1317[linking]
Publication state
Published
Issued date
01/1978
Volume
38
Number
1
Pages
135-147
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
The DNAs of rabbitpox, vaccinia, Cowpox, ectromelia and fowl pox virus, all
grown in the same host system, were cleaved with restriction endonucleases
HindIII, BarnI and EcoRI. The resulting digestion products were separated by
electrophoresis on agarose slab gels. From the similarities and dissimilarities of
the migration profiles obtained, it was concluded that vaccinia and rabbitpox virus
are genetically the most closely related viruses investigated, although their DNA
cleavage patterns are clearly distinguishable. Cowpox and ectromelia virus both
show about the same degree of relatedness to each other as to rabbitpox and
vaccinia virus. Fowl pox virus, which belongs to the genus Avipoxvirus, contains
the largest genome examined (approx. I6O × IO
6 daltons) and the cleavage patterns
of its DNA show no similarities with those of orthopoxvirus DNAs, thus indicat-
ing a very low degree of genetic relatedness. We believe that restriction analysis is
a useful method for the identification and classification of poxvirus isolates.
Keywords
Animals, Chick Embryo, Culture Techniques, DNA Restriction Enzymes/metabolism, DNA, Viral/analysis, Ectromelia virus/analysis, Fowlpox virus/analysis, Humans, Infant, Newborn, Molecular Weight, Nucleic Acid Conformation, Poxviridae/analysis, Vaccinia virus/analysis
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 10:43
Last modification date
20/08/2019 14:56
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