Phytochrome-mediated inhibition of shade avoidance involves degradation of growth-promoting bHLH transcription factors

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State: Public
Version: author
Serval ID
serval:BIB_938A0E4E5B34
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Phytochrome-mediated inhibition of shade avoidance involves degradation of growth-promoting bHLH transcription factors
Journal
Plant Journal
Author(s)
Lorrain S., Allen T., Duek P. D., Whitelam G. C., Fankhauser C.
ISSN
0960-7412
Publication state
Published
Issued date
2008
Peer-reviewed
Oui
Volume
53
Number
2
Pages
312-323
Language
english
Abstract
Plant growth and development are particularly sensitive to changes in the light environment and especially to vegetational shading. The shade-avoidance response is mainly controlled by the phytochrome photoreceptors. In Arabidopsis, recent studies have identified several related bHLH class transcription factors (PIF, for phytochrome-interacting factors) as important components in phytochrome signaling. In addition to a related bHLH domain, most of the PIFs contain an active phytochrome binding (APB) domain that mediates their interaction with light-activated phytochrome B (phyB). Here we show that PIF4 and PIF5 act early in the phytochrome signaling pathways to promote the shade-avoidance response. PIF4 and PIF5 accumulate to high levels in the dark, are selectively degraded in response to red light, and remain at high levels under shade-mimicking conditions. Degradation of these transcription factors is preceded by phosphorylation, requires the APB domain and is sensitive to inhibitors of the proteasome, suggesting that PIF4 and PIF5 are degraded upon interaction with light-activated phyB. Our data suggest that, in dense vegetation, which is rich in far-red light, shade avoidance is triggered, at least partially, as a consequence of reduced phytochrome-mediated degradation of transcription factors such as PIF4 and PIF5. Consistent with this idea, the constitutive shade-avoidance phenotype of phyB mutants partially reverts in the absence of PIF4 and PIF5
Keywords
Arabidopsis , Arabidopsis Proteins , Basic Helix-Loop-Helix Transcription Factors , Gene Expression Regulation,Plant , Genes,Plant , Genomics , Growth , Light , metabolism , Phenotype , Phosphorylation , Phytochrome , Phytochrome B , Proteasome Endopeptidase Complex , Proteins , Switzerland , Transcription Factors
Pubmed
Web of science
Create date
29/01/2009 23:12
Last modification date
20/08/2019 15:56
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