Detection of ASC Oligomerization by Western Blotting.

Details

Serval ID
serval:BIB_93717B76B904
Type
Article: article from journal or magazin.
Collection
Publications
Title
Detection of ASC Oligomerization by Western Blotting.
Journal
Bio-Protocol
Author(s)
Lugrin J., Martinon F.
ISSN
2331-8325 (Print)
ISSN-L
2331-8325
Publication state
Published
Issued date
2017
Peer-reviewed
Oui
Volume
7
Number
10
Pages
NA
Language
english
Abstract
The apoptosis-associated speck-like protein with a caspase-recruitment domain (ASC) adaptor protein bridges inflammasome sensors and caspase-1. Upon inflammasome activation, ASC nucleates in a prion-like manner into a large and single platform responsible for the recruitment and the activation of caspase-1. Active caspase-1 will in turn promote the proteolytic maturation of the pro-inflammatory cytokine IL-1β. ASC oligomerization is a direct evidence for inflammasome activation and its detection allows a read-out independent of caspase-1 and IL-1β. This protocol describes how to detect the oligomerization of ASC by Western blot.

Keywords
Auto-inflammation, Biochemistry, Caspase-1, Inflammasome, Pyroptosis
Pubmed
Create date
09/11/2017 18:31
Last modification date
03/03/2018 19:34
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