Metabolic oscillations on the circadian time scale in <i>Drosophila</i> cells lacking clock genes.
Details
State: Public
Version: Final published version
Serval ID
serval:BIB_92C579221F0E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Metabolic oscillations on the circadian time scale in <i>Drosophila</i> cells lacking clock genes.
Journal
Molecular Systems Biology
ISSN
1744-4292 (Electronic)
ISSN-L
1744-4292
Publication state
Published
Issued date
2018
Peer-reviewed
Oui
Volume
14
Number
8
Pages
e8376
Language
english
Abstract
Circadian rhythms are cell-autonomous biological oscillations with a period of about 24 h. Current models propose that transcriptional feedback loops are the primary mechanism for the generation of circadian oscillations. Within this framework, <i>Drosophila</i> S2 cells are regarded as "non-rhythmic" cells, as they do not express several canonical circadian components. Using an unbiased multi-omics approach, we made the surprising discovery that <i>Drosophila</i> S2 cells do in fact display widespread daily rhythms. Transcriptomics and proteomics analyses revealed that hundreds of genes and their products, and in particular metabolic enzymes, are rhythmically expressed in a 24-h cycle. Metabolomics analyses extended these findings and demonstrate that central carbon metabolism and amino acid metabolism are core metabolic pathways driven by protein rhythms. We thus demonstrate that 24-h metabolic oscillations, coupled to gene and protein cycles, take place in nucleated cells without the contribution of any known circadian regulators. These results therefore suggest a reconsideration of existing models of the clockwork in <i>Drosophila</i> and other eukaryotic systems.
Keywords
circadian, clocks, metabolomics, proteomics, transcriptomics
Pubmed
Web of science
Open Access
Yes
Create date
03/10/2018 12:25
Last modification date
20/08/2019 14:55