Metabolic oscillations on the circadian time scale in <i>Drosophila</i> cells lacking clock genes.

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State: Serval
Version: Final published version
Serval ID
serval:BIB_92C579221F0E
Type
Article: article from journal or magazin.
Collection
Publications
Title
Metabolic oscillations on the circadian time scale in <i>Drosophila</i> cells lacking clock genes.
Journal
Molecular Systems Biology
Author(s)
Rey G., Milev N.B., Valekunja U.K., Ch R., Ray S., Silva Dos Santos M., Nagy A.D., Antrobus R., MacRae J.I., Reddy A.B.
ISSN
1744-4292 (Electronic)
ISSN-L
1744-4292
Publication state
Published
Issued date
2018
Peer-reviewed
Oui
Volume
14
Number
8
Pages
e8376
Language
english
Abstract
Circadian rhythms are cell-autonomous biological oscillations with a period of about 24 h. Current models propose that transcriptional feedback loops are the primary mechanism for the generation of circadian oscillations. Within this framework, <i>Drosophila</i> S2 cells are regarded as "non-rhythmic" cells, as they do not express several canonical circadian components. Using an unbiased multi-omics approach, we made the surprising discovery that <i>Drosophila</i> S2 cells do in fact display widespread daily rhythms. Transcriptomics and proteomics analyses revealed that hundreds of genes and their products, and in particular metabolic enzymes, are rhythmically expressed in a 24-h cycle. Metabolomics analyses extended these findings and demonstrate that central carbon metabolism and amino acid metabolism are core metabolic pathways driven by protein rhythms. We thus demonstrate that 24-h metabolic oscillations, coupled to gene and protein cycles, take place in nucleated cells without the contribution of any known circadian regulators. These results therefore suggest a reconsideration of existing models of the clockwork in <i>Drosophila</i> and other eukaryotic systems.
Keywords
circadian, clocks, metabolomics, proteomics, transcriptomics
Pubmed
Web of science
Open Access
Yes
Create date
03/10/2018 13:25
Last modification date
08/05/2019 22:07
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