Broad spectrum developmental role of Brachypodium AUX1.

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Version: Final published version
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Serval ID
serval:BIB_92BAD1EBBD0B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Broad spectrum developmental role of Brachypodium AUX1.
Journal
The New phytologist
Author(s)
van der Schuren A., Voiniciuc C., Bragg J., Ljung K., Vogel J., Pauly M., Hardtke C.S.
ISSN
1469-8137 (Electronic)
ISSN-L
0028-646X
Publication state
Published
Issued date
09/2018
Peer-reviewed
Oui
Volume
219
Number
4
Pages
1216-1223
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
Publication Status: ppublish
Abstract
Targeted cellular auxin distribution is required for morphogenesis and adaptive responses of plant organs. In Arabidopsis thaliana (Arabidopsis), this involves the prototypical auxin influx facilitator AUX1 and its LIKE-AUX1 (LAX) homologs, which act partially redundantly in various developmental processes. Interestingly, AUX1 and its homologs are not strictly essential for the Arabidopsis life cycle. Indeed, aux1 lax1 lax2 lax3 quadruple knock-outs are mostly viable and fertile, and strong phenotypes are only observed at low penetrance. Here we investigated the Brachypodium distachyon (Brachypodium) AUX1 homolog BdAUX1 by genetic, cell biological and physiological analyses. We report that BdAUX1 is essential for Brachypodium development. Bdaux1 loss-of-function mutants are dwarfs with aberrant flower development, and consequently infertile. Moreover, they display a counter-intuitive root phenotype. Although Bdaux1 roots are agravitropic as expected, in contrast to Arabidopsis aux1 mutants they are dramatically longer than wild type roots because of exaggerated cell elongation. Interestingly, this correlates with higher free auxin content in Bdaux1 roots. Consistently, their cell wall characteristics and transcriptome signature largely phenocopy other Brachypodium mutants with increased root auxin content. Our results imply fundamentally different wiring of auxin transport in Brachypodium roots and reveal an essential role of BdAUX1 in a broad spectrum of developmental processes, suggesting a central role for AUX1 in pooideae.
Keywords
Brachypodium/genetics, Brachypodium/growth & development, Brachypodium/metabolism, Gene Expression Regulation, Plant, Gravitropism/physiology, Mutation/genetics, Phenotype, Plant Development, Plant Proteins/genetics, Plant Proteins/metabolism, Plant Roots/anatomy & histology, Plant Shoots/anatomy & histology, AUX1, Brachypodium, auxin, monocotyledon, seminal root
Pubmed
Web of science
Open Access
Yes
Create date
29/06/2018 17:00
Last modification date
28/09/2019 6:08
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