Rearrangements of minisatellites in the human telomerase reverse transcriptase gene are not correlated with its expression in colon carcinomas
Details
Serval ID
serval:BIB_918226DFA05B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Rearrangements of minisatellites in the human telomerase reverse transcriptase gene are not correlated with its expression in colon carcinomas
Journal
Oncogene
ISSN
0950-9232 (Print)
Publication state
Published
Issued date
05/2001
Volume
20
Number
20
Pages
2600-5
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May 3
Research Support, Non-U.S. Gov't --- Old month value: May 3
Abstract
Telomerase activation is crucial in human carcinogenesis. The limiting component of telomerase, the catalytic subunit (hTERT), is undetectable in normal somatic cells but present in most tumor cells, including the earliest stages of colon carcinoma. The mechanisms involved in the differential expression in normal and tumor cells are not understood. In normal cells hTERT expression is shut down by a repressor, and upregulation could be a consequence of cis-acting changes in the hTERT gene, making it resistant to repression. We have identified a polymorphic and a monomorphic minisatellite in the second intron of the hTERT gene, and polymorphic one in intron 6. The polymorphic minisatellite in intron 2 contains binding sites for c-Myc, which has been shown to upregulate hTERT transcription. Screening colon carcinoma DNAs for rearrangements of hTERT minisatellites we detected no changes in 33 samples from tumors, most of which express hTERT. This indicates that size rearrangements of the hTERT minisatellites are not required for telomerase expression in colon carcinomas. Minor changes and one LOH were seen in five tumors.
Keywords
Base Sequence
Colonic Neoplasms/*enzymology/*genetics
DNA/genetics
DNA, Neoplasm/genetics
DNA-Binding Proteins
Gene Rearrangement
Humans
Microsatellite Repeats/*genetics
Molecular Sequence Data
*Polymorphism, Genetic
*Rna
Telomerase/biosynthesis/*genetics
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 13:59
Last modification date
20/08/2019 15:54