Macrophage migration inhibitory factor deficiency leads to age-dependent impairment of glucose homeostasis in mice.
Details
Serval ID
serval:BIB_9176461B94ED
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Macrophage migration inhibitory factor deficiency leads to age-dependent impairment of glucose homeostasis in mice.
Journal
Journal of Endocrinology
ISSN
1479-6805[electronic], 0022-0795[linking]
Publication state
Published
Issued date
2010
Volume
206
Number
3
Pages
297-306
Language
english
Abstract
Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine produced by many cells and tissues including pancreatic beta-cells, liver, skeletal muscle, and adipocytes. This study investigates the potential role of MIF in carbohydrate homeostasis in a physiological setting outside of severe inflammation, utilizing Mif knockout (MIF-/-) mice. Compared with wild-type (WT) mice, MIF-/- mice had a lower body weight, from birth until 4 months of age, but subsequently gained weight faster, resulting in a higher body weight at 12 months of age. The lower weight in young mice was related to a higher energy expenditure, and the higher weight in older mice was related to an increased food intake and a higher fat mass. Fasting blood insulin level was higher in MIF-/- mice compared with WT mice at any age. After i.p. glucose injection, the elevation of blood insulin level was higher in MIF-/- mice compared with WT mice, at 2 months of age, but was lower in 12-month-old MIF-/- mice. As a result, the glucose clearance during intraperitoneal glucose tolerance tests was higher in MIF-/- mice compared with WT mice until 4 months of age, and was lower in 12-month-old MIF-/- mice. Insulin resistance was estimated (euglycemic-hyperinsulinemic clamp tests), and the phosphorylation activity of AKT was similar in MIF-/- mice and WT mice. In conclusion, this mouse model provides evidence for the role of MIF in the control of glucose homeostasis.
Keywords
Age Factors, Analysis of Variance, Animals, Blood Glucose/metabolism, Blotting, Western, Body Composition/physiology, Body Weight/physiology, Energy Metabolism/physiology, Glucose Clamp Technique, Glucose Tolerance Test, Homeostasis/physiology, Insulin/metabolism, Insulin Resistance/physiology, Leptin/blood, Lipids/blood, Macrophage Migration-Inhibitory Factors/genetics, Macrophage Migration-Inhibitory Factors/metabolism, Mice, Mice, Inbred C57BL, Mice, Knockout, Motor Activity/physiology, Reverse Transcriptase Polymerase Chain Reaction, Statistics, Nonparametric
Pubmed
Web of science
Open Access
Yes
Create date
27/10/2010 15:17
Last modification date
20/08/2019 15:54