Microfluidic T Cell Selection by Cellular Avidity.

Details

Serval ID
serval:BIB_9101DBCC66E2
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Microfluidic T Cell Selection by Cellular Avidity.
Journal
Advanced healthcare materials
Author(s)
Ashby J.F., Schmidt J., Kc N., Kurum A., Koch C., Harari A., Tang L., Au S.H.
ISSN
2192-2659 (Electronic)
ISSN-L
2192-2640
Publication state
Published
Issued date
08/2022
Peer-reviewed
Oui
Volume
11
Number
16
Pages
e2200169
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
No T cell receptor (TCR) T cell therapies have obtained clinical approval. The lack of strategies capable of selecting and recovering potent T cell candidates may be a contributor to this. Existing protocols for selecting TCR T cell clones for cell therapies such as peptide multimer methods have provided effective measurements on TCR affinities. However, these methods lack the ability to measure the collective strength of intercellular interactions (i.e., cellular avidity) and markers of T cell activation such as immunological synapse formation. This study describes a novel microfluidic fluid shear stress-based approach to identify and recover highly potent T cell clones based on the cellular avidity between living T cells and tumor cells. This approach is capable of probing approximately up to 10 000 T cell-tumor cell interactions per run and can recover potent T cells with up to 100% purity from mixed populations of T cells within 30 min. Markers of cytotoxicity, activation, and avidity persist when recovered high cellular avidity T cells are subsequently exposed to fresh tumor cells. These results demonstrate how microfluidic probing of cellular avidity may fast track the therapeutic T cell selection process and move the authors closer to precision cancer immunotherapy.
Keywords
Lymphocyte Activation, Microfluidics, Peptides, Receptors, Antigen, T-Cell, T-Lymphocytes, T cell receptors, avidity, cytotoxicity, immunotherapy, microfluidics, shear stress, synapses
Pubmed
Web of science
Open Access
Yes
Create date
07/08/2023 15:47
Last modification date
13/10/2023 6:01
Usage data