Down selecting adjuvanted vaccine formulations: a comparative method for harmonized evaluation.

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State: Public
Version: Final published version
Serval ID
serval:BIB_90B6C5F245AB
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Down selecting adjuvanted vaccine formulations: a comparative method for harmonized evaluation.
Journal
BMC immunology
Author(s)
Younis S.Y., Barnier-Quer C., Heuking S., Sommandas V., Brunner L., Vd Werff N., Dubois P., Friede M., Kocken C., Collin N., Remarque E.
ISSN
1471-2172 (Electronic)
ISSN-L
1471-2172
Publication state
Published
Issued date
31/01/2018
Peer-reviewed
Oui
Volume
19
Number
1
Pages
6
Language
english
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
The need for rapid and accurate comparison of panels of adjuvanted vaccine formulations and subsequent rational down selection, presents several challenges for modern vaccine development. Here we describe a method which may enable vaccine and adjuvant developers to compare antigen/adjuvant combinations in a harmonized fashion. Three reference antigens: Plasmodium falciparum apical membrane antigen 1 (AMA1), hepatitis B virus surface antigen (HBsAg), and Mycobacterium tuberculosis antigen 85A (Ag85A), were selected as model antigens and were each formulated with three adjuvants: aluminium oxyhydroxide, squalene-in-water emulsion, and a liposome formulation mixed with the purified saponin fraction QS21.
The nine antigen/adjuvant formulations were assessed for stability and immunogenicity in mice in order to provide benchmarks against which other formulations could be compared, in order to assist subsequent down selection of adjuvanted vaccines. Furthermore, mouse cellular immune responses were analyzed by measuring IFN-γ and IL-5 production in splenocytes by ELISPOT, and humoral responses were determined by antigen-specific ELISA, where levels of total IgG, IgG1, IgG2b and IgG2c in serum samples were determined.
The reference antigens and adjuvants described in this study, which span a spectrum of immune responses, are of potential use as tools to act as points of reference in vaccine development studies. The harmonized methodology described herein may be used as a tool for adjuvant/antigen comparison studies.
Keywords
Acyltransferases/immunology, Adjuvants, Immunologic/analysis, Animals, Antigens, Bacterial/immunology, Antigens, Protozoan/immunology, Enzyme-Linked Immunosorbent Assay/methods, Enzyme-Linked Immunospot Assay/methods, Hepatitis B Surface Antigens/immunology, Immunization, Immunoglobulin G/blood, Immunoglobulin G/immunology, Interferon-gamma/immunology, Interferon-gamma/metabolism, Interleukin-5/immunology, Interleukin-5/metabolism, Membrane Proteins/immunology, Mice, Inbred C57BL, Protozoan Proteins/immunology, Reproducibility of Results, Spleen/cytology, Spleen/immunology, Spleen/metabolism, Vaccines/analysis, Vaccines/immunology, AMA1, Adjuvants, Ag85A, Aluminium oxyhydroxide, HBsAg, Hepatitis B, Plasmodium falciparum, QS21, Squalene-in-water SWE, Tuberculosis
Pubmed
Web of science
Open Access
Yes
Create date
08/02/2018 20:44
Last modification date
20/08/2019 15:54
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