Targeting Mutated Plus Germline Epitopes Confers Pre-clinical Efficacy of an Instantly Formulated Cancer Nano-Vaccine.

Details

Serval ID
serval:BIB_90783492E7F6
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Targeting Mutated Plus Germline Epitopes Confers Pre-clinical Efficacy of an Instantly Formulated Cancer Nano-Vaccine.
Journal
Frontiers in immunology
Author(s)
Mohsen M.O., Vogel M., Riether C., Muller J., Salatino S., Ternette N., Gomes A.C., Cabral-Miranda G., El-Turabi A., Ruedl C., Kundig T.M., Dermime S., Knuth A., Speiser D.E., Bachmann M.F.
ISSN
1664-3224 (Electronic)
ISSN-L
1664-3224
Publication state
Published
Issued date
2019
Peer-reviewed
Oui
Volume
10
Pages
1015
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Personalized cancer vaccines hold promises for future cancer therapy. Targeting neoantigens is perceived as more beneficial compared to germline, non-mutated antigens. However, it is a practical challenge to identify and vaccinate patients with neoantigens. Here we asked whether two neoantigens are sufficient, and whether the addition of germline antigens would enhance the therapeutic efficacy. We developed and used a personalized cancer nano-vaccine platform based on virus-like particles loaded with toll-like receptor ligands. We generated three sets of multi-target vaccines (MTV) to immunize against the aggressive B16F10 murine melanoma: one set based on germline epitopes (GL-MTV) identified by immunopeptidomics, another set based on mutated epitopes (Mutated-MTV) predicted by whole exome sequencing and a last set combines both germline and mutated epitopes (Mix-MTV). Our results demonstrate that both germline and mutated epitopes induced protection but the best therapeutic effect was achieved with the combination of both. Our platform is based on Cu-free click chemistry used for peptide-VLP coupling, thus enabling bedside production of a personalized cancer vaccine, ready for clinical translation.
Keywords
germline, melanoma, mutated, neoantigen, personalized, vaccine, virus-like particles
Pubmed
Web of science
Open Access
Yes
Create date
17/06/2019 18:00
Last modification date
20/08/2019 15:53
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