Arabidopsis lox3 lox4 double mutants are male sterile and defective in global proliferative arrest.

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Serval ID
serval:BIB_9074DD3E3C5A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Arabidopsis lox3 lox4 double mutants are male sterile and defective in global proliferative arrest.
Journal
Plant Molecular Biology
Author(s)
Caldelari D., Wang G., Farmer E.E., Dong X.
ISSN
1573-5028 (Electronic)
ISSN-L
0167-4412
Publication state
Published
Issued date
2011
Volume
75
Number
1-2
Pages
25-33
Language
english
Abstract
Fertility and flower development are both controlled in part by jasmonates, fatty acid-derived mediators produced via the activity of 13-lipoxygenases (13-LOXs). The Arabidopsis thaliana Columbia-0 reference genome is predicted to encode four of these enzymes and it is already known that one of these, LOX2, is dispensable for fertility. In this study, the roles of the other three 13-LOXs (LOX3, LOX4 and LOX6) were investigated in single and double mutants. Four independent lox3 lox4 double mutants assembled with different mutated lox3 and lox4 alleles had fully penetrant floral phenotypes, displaying abnormal anther maturation and defective dehiscence. The plants were no longer self-fertile and pollen was not viable. Fertility in the double mutant was restored genetically by complementation with either the LOX3 or the LOX4 cDNAs and biochemically with exogenous jasmonic acid. Furthermore, deficiency in LOX3 and LOX4 causes developmental dysfunctions, compared to wild type; lox3 lox4 double mutants are taller and develop more inflorescence shoots and flowers. Further analysis revealed that developmental arrest in the lox3 lox4 inflorescence occurs with the production of an abnormal carpelloid flower. This distinguishes lox3 lox4 mutants from the wild type where developmentally typical flower buds are the terminal inflorescence structures observed in both the laboratory and in nature. Our studies of lox3 lox4 as well as other jasmonic acid biosynthesis and perception mutants show that this plant hormone is not only required for male fertility but also involved in global proliferative arrest.
Keywords
Arabidopsis/genetics, Arabidopsis/growth & development, Arabidopsis Proteins/genetics, Arabidopsis Proteins/metabolism, Cell Proliferation, Cyclopentanes/metabolism, Flowers/genetics, Flowers/growth & development, Genetic Complementation Test, Isoenzymes/genetics, Isoenzymes/metabolism, Lipoxygenase/genetics, Lipoxygenase/metabolism, Mutation, Oxylipins/metabolism, Plant Infertility/genetics, Pollen/cytology, Pollen/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
18/01/2011 15:39
Last modification date
01/10/2019 7:18
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