Cell wall synthesis and remodelling dynamics determine division site architecture and cell shape in Escherichia coli.
Details
Serval ID
serval:BIB_8F44D18EADD2
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cell wall synthesis and remodelling dynamics determine division site architecture and cell shape in Escherichia coli.
Journal
Nature microbiology
ISSN
2058-5276 (Electronic)
ISSN-L
2058-5276
Publication state
Published
Issued date
10/2022
Peer-reviewed
Oui
Volume
7
Number
10
Pages
1621-1634
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
Publication Status: ppublish
Publication Status: ppublish
Abstract
The bacterial division apparatus catalyses the synthesis and remodelling of septal peptidoglycan (sPG) to build the cell wall layer that fortifies the daughter cell poles. Understanding of this essential process has been limited by the lack of native three-dimensional views of developing septa. Here, we apply state-of-the-art cryogenic electron tomography (cryo-ET) and fluorescence microscopy to visualize the division site architecture and sPG biogenesis dynamics of the Gram-negative bacterium Escherichia coli. We identify a wedge-like sPG structure that fortifies the ingrowing septum. Experiments with strains defective in sPG biogenesis revealed that the septal architecture and mode of division can be modified to more closely resemble that of other Gram-negative (Caulobacter crescentus) or Gram-positive (Staphylococcus aureus) bacteria, suggesting that a conserved mechanism underlies the formation of different septal morphologies. Finally, analysis of mutants impaired in amidase activation (ΔenvC ΔnlpD) showed that cell wall remodelling affects the placement and stability of the cytokinetic ring. Taken together, our results support a model in which competition between the cell elongation and division machineries determines the shape of cell constrictions and the poles they form. They also highlight how the activity of the division system can be modulated to help generate the diverse array of shapes observed in the bacterial domain.
Keywords
Amidohydrolases, Cell Division, Cell Shape, Cell Wall, Escherichia coli/physiology, Peptidoglycan
Pubmed
Web of science
Open Access
Yes
Create date
22/07/2024 11:30
Last modification date
26/07/2024 15:25